The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters

Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the...

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Main Author:	Wahab, Wan Nor Najmiyah Wan Abdul
Format:	Thesis
Language:	English
Published:	2017
Subjects:	RC Internal medicine
Online Access:	http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf
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spelling	my-usm-ep.456822019-10-17T08:35:53Z The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters 2017 Wahab, Wan Nor Najmiyah Wan Abdul RC Internal medicine Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the frequency of double or triple protein expression by using immunohistochemistry (IHC) and relating the result with clinicopathological features and cell of origin (COO) classification. Material and methods: We conducted a cross sectional study by using 29 archived formalin-fixed paraffin embedded tissue blocks of DLBCL. All the samples were evaluated for expression of MYC, BCL2 and BCL6 by IHC. In addition, the subgrouping of COO DLBCL was determined by expression of CD10, BCL6 and MUM1 based on Hans classification. Result: Among the 29 cases, MYC, BCL2 and BCL6 proteins were detected in 72.4%, 62.1% and 62.1% of patients, respectively. Concurrent expression (MYC positive/BCL2 positive and/or BCL6 positive) was present in 58.6% of patients. 34.5% were categorized as germinal centre like (GCB) subgroup and 65.5% were categorized as non germinal centre like (non-GCB) subgroup. Among the clinicopathological features, the double/triple protein expression lymphoma was significantly associated with elevated LDH level (p=0.018), IPI score (p=0.003), Ann Arbor stage (p=0.011) and complete response rate (p=0.011Conclusion: Double/triple protein lymphoma was strongly associated more adverse clinical risk factors. Thus, analyses of MYC, BCL2 and BCL6 expression by IHC represents a rapid and inexpensive approach to risk-stratify patients with DLBCL at diagnosis. 2017 Thesis http://eprints.usm.my/45682/ http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Wahab, Wan Nor Najmiyah Wan Abdul The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
description	Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the frequency of double or triple protein expression by using immunohistochemistry (IHC) and relating the result with clinicopathological features and cell of origin (COO) classification. Material and methods: We conducted a cross sectional study by using 29 archived formalin-fixed paraffin embedded tissue blocks of DLBCL. All the samples were evaluated for expression of MYC, BCL2 and BCL6 by IHC. In addition, the subgrouping of COO DLBCL was determined by expression of CD10, BCL6 and MUM1 based on Hans classification. Result: Among the 29 cases, MYC, BCL2 and BCL6 proteins were detected in 72.4%, 62.1% and 62.1% of patients, respectively. Concurrent expression (MYC positive/BCL2 positive and/or BCL6 positive) was present in 58.6% of patients. 34.5% were categorized as germinal centre like (GCB) subgroup and 65.5% were categorized as non germinal centre like (non-GCB) subgroup. Among the clinicopathological features, the double/triple protein expression lymphoma was significantly associated with elevated LDH level (p=0.018), IPI score (p=0.003), Ann Arbor stage (p=0.011) and complete response rate (p=0.011Conclusion: Double/triple protein lymphoma was strongly associated more adverse clinical risk factors. Thus, analyses of MYC, BCL2 and BCL6 expression by IHC represents a rapid and inexpensive approach to risk-stratify patients with DLBCL at diagnosis.
format	Thesis
qualification_level	Master's degree
author	Wahab, Wan Nor Najmiyah Wan Abdul
author_facet	Wahab, Wan Nor Najmiyah Wan Abdul
author_sort	Wahab, Wan Nor Najmiyah Wan Abdul
title	The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_short	The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_full	The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_fullStr	The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_full_unstemmed	The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_sort	immunohistochemical studies of double or triple protein lymphoma in diffuse large b-cell lymphoma and their correlation with clinicopathological parameters
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2017
url	http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf
_version_	1747821546040197120

The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters

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