Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans

Clinacanthus nutans (C. nutans) locally known as Sabah snake grass is a perennial herb that has been used traditionally in the treatment of various diseases in Malaysia. The objectives of this study were to determine the phytochemical compounds in the ethanol and aqueous extracts of C. nutans lea...

Full description

Saved in:

Bibliographic Details
Main Author:	Alim, A'attiyyah Ab
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RM Therapeutics Pharmacology
Online Access:	http://eprints.usm.my/45711/1/A%E2%80%99attiyyah%20Ab%20Alim-24%20pages.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.45711
record_format	uketd_dc
spelling	my-usm-ep.457112019-10-23T07:53:04Z Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans 2018 Alim, A'attiyyah Ab RM Therapeutics. Pharmacology Clinacanthus nutans (C. nutans) locally known as Sabah snake grass is a perennial herb that has been used traditionally in the treatment of various diseases in Malaysia. The objectives of this study were to determine the phytochemical compounds in the ethanol and aqueous extracts of C. nutans leaves using Gas Chromatography Mass Spectrometry (GCMS) and assessing their cytotoxic effect of using MTT assay on human gingival fibroblast cell line and then to evaluate their mutagenic effect using Ames test as well as to determine their antimicrobial properties using agar diffusion test and Minimal Inhibitory Concentration (MIC) assays. A total of 53 phytochemical compounds were identified from the extracts of C. nutans leaves. The compounds were classified into several classes: phenols, flavonoids, terpenoids, fatty acids, alkanes and hydrocarbons, esters, ketones, furans, sulfur and nitrogen compounds. In vitro cytotoxic study of ethanol and aqueous extracts of C. nutans leaves in various concentrations (6,400; 3,200; 1,600; 800; 400; 200; 100; 50 and 25 μg/ml) on human gingival fibroblast cell line (HGF-1) using MTT assay did not exhibit any cytotoxic effect. Ames test on four types of S. Typhimurium strains, TA98, TA100, TA1535 and TA1537 treated with ethanol and aqueous extracts of C. nutans leaves did not show any mutagenic effect both in the presence and absence of metabolic activation system (S9) mix based on the number of revertant colonies. Antimicrobial study using agar well diffusion assay showed that the growth of Staphylococcus aureus (S. aureus) and Actinomyces viscosus (A. viscosus) wereinhibited by the ethanol and aqueous extracts of C. nutans leaves. However, the extracts did not exhibit any antimicrobial activity towards Enterococcus faecalis (E. faecalis), Porhyromonas gingivalis (P. gingivalis) and Candida albicans (C. albicans). Ethanol extract of C. nutans leaves showed better antimicrobial activity compared to the aqueous extract of C. nutans leaves against A. viscosus. Ethanol extract of C. nutans leaves showed MIC value of 102,400 μg/ml against A. viscosus and 204,800 μg/ml against S. aureus. Aqueous extract of C. nutans leaves showed MIC values of 204,800 μg/ml against A. viscosus and S. aureus. The results of the current study show that C. nutans leaves could act as a potential source of antimicrobial agent in combating oral pathogens. 2018 Thesis http://eprints.usm.my/45711/ http://eprints.usm.my/45711/1/A%E2%80%99attiyyah%20Ab%20Alim-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RM Therapeutics Pharmacology
spellingShingle	RM Therapeutics Pharmacology Alim, A'attiyyah Ab Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
description	Clinacanthus nutans (C. nutans) locally known as Sabah snake grass is a perennial herb that has been used traditionally in the treatment of various diseases in Malaysia. The objectives of this study were to determine the phytochemical compounds in the ethanol and aqueous extracts of C. nutans leaves using Gas Chromatography Mass Spectrometry (GCMS) and assessing their cytotoxic effect of using MTT assay on human gingival fibroblast cell line and then to evaluate their mutagenic effect using Ames test as well as to determine their antimicrobial properties using agar diffusion test and Minimal Inhibitory Concentration (MIC) assays. A total of 53 phytochemical compounds were identified from the extracts of C. nutans leaves. The compounds were classified into several classes: phenols, flavonoids, terpenoids, fatty acids, alkanes and hydrocarbons, esters, ketones, furans, sulfur and nitrogen compounds. In vitro cytotoxic study of ethanol and aqueous extracts of C. nutans leaves in various concentrations (6,400; 3,200; 1,600; 800; 400; 200; 100; 50 and 25 μg/ml) on human gingival fibroblast cell line (HGF-1) using MTT assay did not exhibit any cytotoxic effect. Ames test on four types of S. Typhimurium strains, TA98, TA100, TA1535 and TA1537 treated with ethanol and aqueous extracts of C. nutans leaves did not show any mutagenic effect both in the presence and absence of metabolic activation system (S9) mix based on the number of revertant colonies. Antimicrobial study using agar well diffusion assay showed that the growth of Staphylococcus aureus (S. aureus) and Actinomyces viscosus (A. viscosus) wereinhibited by the ethanol and aqueous extracts of C. nutans leaves. However, the extracts did not exhibit any antimicrobial activity towards Enterococcus faecalis (E. faecalis), Porhyromonas gingivalis (P. gingivalis) and Candida albicans (C. albicans). Ethanol extract of C. nutans leaves showed better antimicrobial activity compared to the aqueous extract of C. nutans leaves against A. viscosus. Ethanol extract of C. nutans leaves showed MIC value of 102,400 μg/ml against A. viscosus and 204,800 μg/ml against S. aureus. Aqueous extract of C. nutans leaves showed MIC values of 204,800 μg/ml against A. viscosus and S. aureus. The results of the current study show that C. nutans leaves could act as a potential source of antimicrobial agent in combating oral pathogens.
format	Thesis
qualification_level	Master's degree
author	Alim, A'attiyyah Ab
author_facet	Alim, A'attiyyah Ab
author_sort	Alim, A'attiyyah Ab
title	Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
title_short	Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
title_full	Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
title_fullStr	Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
title_full_unstemmed	Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
title_sort	cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/45711/1/A%E2%80%99attiyyah%20Ab%20Alim-24%20pages.pdf
_version_	1747821550916075520

Cytotoxic, mutagenic and oral pathogen related antimicrobial properties of clinacanthus nutans

Similar Items