Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass

In this study, nitrogen was removed from wastewater via partial nitrification using both floccular and granular biomass. The purpose of this study is to investigate the effect of starvation on nitrogen removing bacteria, changes of biomass in term of structure during starvation, and recovery ability...

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Main Author:	Tan, Teong Ee
Format:	Thesis
Language:	English
Published:	2017
Subjects:	T Technology TP155-156 Chemical engineering
Online Access:	http://eprints.usm.my/45755/1/Starvation%20And%20Reactivation%20Of%20Partial%20Nitrifying%20Bacteria%2C%20Comparison%20Between%20Floccular%20And%20Granular%20Biomass.pdf
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spelling	my-usm-ep.457552021-11-17T03:42:16Z Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass 2017-05 Tan, Teong Ee T Technology TP155-156 Chemical engineering In this study, nitrogen was removed from wastewater via partial nitrification using both floccular and granular biomass. The purpose of this study is to investigate the effect of starvation on nitrogen removing bacteria, changes of biomass in term of structure during starvation, and recovery ability of biomass. 2 Sequencing Batch Reactor (SBR) with working volume of 8L were operated to enrich floccular and granular biomass. Both floccular and granular biomass were cultivated using high strength synthetic wastewater in SBR operating under similar parameters except height/diameter (H/D) ratio and settling time. The height/diameter ratio of SBR used to cultivate granular biomass was 10 which was higher than floccular biomass (5). The settling period of granular biomass was stepwise decreased from 5 to 1 minute for the enhancement of granulation. The cultivated granular biomass has an average diameter of 2mm. The long term performance of floccular and granular biomass were consistent and the results showed that partial nitrification was achieved for both floccular and granular biomass. Comparison was made between floccular and granular biomass in starvation, reactivation and energy distribution. Starvation studies (carbon starvation and energy starvation) were carried out on both floccular and granular biomass. The starvation studies showed that carbon starvation (absence of carbon source, only energy source is provided) has lesser impacts in the activity of both floccular and granular biomass as compared to energy starvation (absence of energy source, only carbon source is provided). Granular biomass was found to be more resilient to the starvation as compared to floccular biomass due to slower rate of reduction in ammonium uptake. Furthermore, recovery study was carried out on both floccular and granular biomass after certain period of starvations (both carbon and energy starvations). The recovery study results showed that carbon starved biomass were able to recovery easily as compared to energy starved biomass. Carbon starved granular biomass was found to be more responsive to the recovery as compared to carbon starved floccular biomass. Prolonged recovery was required for energy starved biomass to reactivate. Energy starved granular biomass required shorter period to recover as compared to energy starved floccular biomass. Maintenance energy distribution study was carried out on both carbon starved floccular biomass and granular biomass. It was found out that granular biomass has higher maintenance energy distribution (89%) during normal condition as compared to floccular biomass (69%). The maintenance energy distribution of both floccular and granular biomass were not affected by the starvation (70%). However, it was believed that the disintegration of the granular structure after starvation induced similar behaviour to the floccular biomass, which was probably the reason behind the similar maintenance energy distribution after starvation. 2017-05 Thesis http://eprints.usm.my/45755/ http://eprints.usm.my/45755/1/Starvation%20And%20Reactivation%20Of%20Partial%20Nitrifying%20Bacteria%2C%20Comparison%20Between%20Floccular%20And%20Granular%20Biomass.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T Technology TP155-156 Chemical engineering
spellingShingle	T Technology TP155-156 Chemical engineering Tan, Teong Ee Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
description	In this study, nitrogen was removed from wastewater via partial nitrification using both floccular and granular biomass. The purpose of this study is to investigate the effect of starvation on nitrogen removing bacteria, changes of biomass in term of structure during starvation, and recovery ability of biomass. 2 Sequencing Batch Reactor (SBR) with working volume of 8L were operated to enrich floccular and granular biomass. Both floccular and granular biomass were cultivated using high strength synthetic wastewater in SBR operating under similar parameters except height/diameter (H/D) ratio and settling time. The height/diameter ratio of SBR used to cultivate granular biomass was 10 which was higher than floccular biomass (5). The settling period of granular biomass was stepwise decreased from 5 to 1 minute for the enhancement of granulation. The cultivated granular biomass has an average diameter of 2mm. The long term performance of floccular and granular biomass were consistent and the results showed that partial nitrification was achieved for both floccular and granular biomass. Comparison was made between floccular and granular biomass in starvation, reactivation and energy distribution. Starvation studies (carbon starvation and energy starvation) were carried out on both floccular and granular biomass. The starvation studies showed that carbon starvation (absence of carbon source, only energy source is provided) has lesser impacts in the activity of both floccular and granular biomass as compared to energy starvation (absence of energy source, only carbon source is provided). Granular biomass was found to be more resilient to the starvation as compared to floccular biomass due to slower rate of reduction in ammonium uptake. Furthermore, recovery study was carried out on both floccular and granular biomass after certain period of starvations (both carbon and energy starvations). The recovery study results showed that carbon starved biomass were able to recovery easily as compared to energy starved biomass. Carbon starved granular biomass was found to be more responsive to the recovery as compared to carbon starved floccular biomass. Prolonged recovery was required for energy starved biomass to reactivate. Energy starved granular biomass required shorter period to recover as compared to energy starved floccular biomass. Maintenance energy distribution study was carried out on both carbon starved floccular biomass and granular biomass. It was found out that granular biomass has higher maintenance energy distribution (89%) during normal condition as compared to floccular biomass (69%). The maintenance energy distribution of both floccular and granular biomass were not affected by the starvation (70%). However, it was believed that the disintegration of the granular structure after starvation induced similar behaviour to the floccular biomass, which was probably the reason behind the similar maintenance energy distribution after starvation.
format	Thesis
qualification_level	Master's degree
author	Tan, Teong Ee
author_facet	Tan, Teong Ee
author_sort	Tan, Teong Ee
title	Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
title_short	Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
title_full	Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
title_fullStr	Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
title_full_unstemmed	Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass
title_sort	starvation and reactivation of partial nitrifying bacteria, comparison between floccular and granular biomass
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2017
url	http://eprints.usm.my/45755/1/Starvation%20And%20Reactivation%20Of%20Partial%20Nitrifying%20Bacteria%2C%20Comparison%20Between%20Floccular%20And%20Granular%20Biomass.pdf
_version_	1747821559002693632

Starvation And Reactivation Of Partial Nitrifying Bacteria, Comparison Between Floccular And Granular Biomass

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