Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients

Introduction Vascular endothelial growth factor (VEGF) has been postulated to play a role in the pathogenesis and progression of diabetic retinopathy (DR). Thus, detection of VEGF level in ocular tissue may perhaps provide insight into a role of VEGF in pathogenesis of DR. Objective: The object...

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Main Author:	Jeat, Ang Wen
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RC648-665 Diseases of the endocrine glands Clinical endocrinology
Online Access:	http://eprints.usm.my/45808/1/Dr.%20Ang%20Wen%20Jeat-24%20pages.pdf
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spelling	my-usm-ep.458082019-12-01T08:00:03Z Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients 2018 Jeat, Ang Wen RC648-665 Diseases of the endocrine glands. Clinical endocrinology Introduction Vascular endothelial growth factor (VEGF) has been postulated to play a role in the pathogenesis and progression of diabetic retinopathy (DR). Thus, detection of VEGF level in ocular tissue may perhaps provide insight into a role of VEGF in pathogenesis of DR. Objective: The objective of this study was to evaluate the VEGF in serum and tears between different type of DR severity among type 2 diabetes mellitus (DM) patients and to determine the correlation between serum and tears VEGF levels. Methods: A comparative cross-sectional study was conducted at a tertiary hospital in Malaysia, Hospital Universiti Sains Malaysia (USM) between August 2016 and May 2018 involving type 2 DM patients with different status of DR [no DR, non-proliferative DR (NPDR), proliferative DR (PDR)]. Tear samples were collected using no.41 Whatman filter paper (Schirmer’s strips) and 5 mls of blood sample were drawn by venous puncture. VEGF levels in tears and serum were measured by enzyme-linked immunosorbent assay. ixResults: A total of 88 type 2 DM patients (no DR: 30 patients, NPDR: 28 patients, PDR: 30 patients) were included into the study. Mean tear VEGF were significantly higher in NPDR and PDR groups (114.9 SD 8.6 pg/mL and 149.5 SD 10.4 pg/mL respectively) as compared to no DR (41.2 SD 11.3 pg/mL, p < 0.001). There was no significant difference of mean serum VEGF between the three groups. There was a fair correlation between serum and tears VEGF (p = 0.015, r = 0.263). Conclusion: Among type 2 DM patients, VEGF levels in tears were higher amongst patients with DR compared to those without DR. Tear VEGF levels were also significantly associated to the severity of DR. There was a fair correlation between serum and tears VEGF. Detection of VEGF in tears is a good non-invasive predictor test for severity of DR. A large cohort study is needed for further evaluation. 2018 Thesis http://eprints.usm.my/45808/ http://eprints.usm.my/45808/1/Dr.%20Ang%20Wen%20Jeat-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC648-665 Diseases of the endocrine glands Clinical endocrinology
spellingShingle	RC648-665 Diseases of the endocrine glands Clinical endocrinology Jeat, Ang Wen Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
description	Introduction Vascular endothelial growth factor (VEGF) has been postulated to play a role in the pathogenesis and progression of diabetic retinopathy (DR). Thus, detection of VEGF level in ocular tissue may perhaps provide insight into a role of VEGF in pathogenesis of DR. Objective: The objective of this study was to evaluate the VEGF in serum and tears between different type of DR severity among type 2 diabetes mellitus (DM) patients and to determine the correlation between serum and tears VEGF levels. Methods: A comparative cross-sectional study was conducted at a tertiary hospital in Malaysia, Hospital Universiti Sains Malaysia (USM) between August 2016 and May 2018 involving type 2 DM patients with different status of DR [no DR, non-proliferative DR (NPDR), proliferative DR (PDR)]. Tear samples were collected using no.41 Whatman filter paper (Schirmer’s strips) and 5 mls of blood sample were drawn by venous puncture. VEGF levels in tears and serum were measured by enzyme-linked immunosorbent assay. ixResults: A total of 88 type 2 DM patients (no DR: 30 patients, NPDR: 28 patients, PDR: 30 patients) were included into the study. Mean tear VEGF were significantly higher in NPDR and PDR groups (114.9 SD 8.6 pg/mL and 149.5 SD 10.4 pg/mL respectively) as compared to no DR (41.2 SD 11.3 pg/mL, p < 0.001). There was no significant difference of mean serum VEGF between the three groups. There was a fair correlation between serum and tears VEGF (p = 0.015, r = 0.263). Conclusion: Among type 2 DM patients, VEGF levels in tears were higher amongst patients with DR compared to those without DR. Tear VEGF levels were also significantly associated to the severity of DR. There was a fair correlation between serum and tears VEGF. Detection of VEGF in tears is a good non-invasive predictor test for severity of DR. A large cohort study is needed for further evaluation.
format	Thesis
qualification_level	Master's degree
author	Jeat, Ang Wen
author_facet	Jeat, Ang Wen
author_sort	Jeat, Ang Wen
title	Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
title_short	Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
title_full	Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
title_fullStr	Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
title_full_unstemmed	Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
title_sort	evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/45808/1/Dr.%20Ang%20Wen%20Jeat-24%20pages.pdf
_version_	1747821571244818432

Evaluation of vascular endothelial growth factor level in tear and serum among diabetic patients

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