Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan

Edible bird nest (EBN) is one of the natural products that have high therapeutic potential and pharmacological properties. Therefore, this study aimed to determine the toxicity and antioxidants activity on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan (Tanah M...

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Main Author:	Shaheran, Anish Ameera
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RM Therapeutics Pharmacology
Online Access:	http://eprints.usm.my/45815/1/Dr.%20Anish%20Ameera-24%20pages.pdf
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id	my-usm-ep.45815
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spelling	my-usm-ep.458152019-12-04T02:39:42Z Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan 2018-01 Shaheran, Anish Ameera RM Therapeutics. Pharmacology Edible bird nest (EBN) is one of the natural products that have high therapeutic potential and pharmacological properties. Therefore, this study aimed to determine the toxicity and antioxidants activity on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan (Tanah Merah, Tumpat and Jeli). The samples were extracted using three solvents which are distilled water, sodium hydroxide and hydrochloric acid. The toxicity of the EBN samples with different solvent of extraction were tested using MTT assay against cancerous (HeLa and MCF-7) and non-cancerous cell line (SVG p12). Minerals content in EBN was studied using inductively couple plasma mass spectrometry (ICP-MS) and the other toxicity test was brine shrimp lethality assay (BSLA) for LC50 values. The antioxidants activity was tested on EBN samples extracts using DPPH radical scavenging assay and Ferric reducing power assay (FRAP). EBN samples extracts showed higher inhibition activity against MCF- 7 cell line. Meanwhile, three samples of EBN extracts induced cell proliferation on SVG cell line as the IC50 value more than 100%. Minerals content in all EBN samples were below the maximum regulatory limit as suggested by FDA, WHO and SIRIM guidelines except sodium content that slightly higher than 2 ppm. The results showed no LC50 value was determine on EBN against brine shrimp. The antioxidants activity using DPPH and FRAP assay showed that NaOH extracts from three sample EBN had the lowest IC50 values and the highest reducing power. Thus, the results finding showed that the EBN samples extracts can be considered as cancer-preventive agents and are relatively safe to be consumed. Extracts of EBN samples can be considered asantioxidants agent. However, further validation have to be performed to understand the active compounds that poses safety, toxicity and antioxidants activity of EBN. 2018-01 Thesis http://eprints.usm.my/45815/ http://eprints.usm.my/45815/1/Dr.%20Anish%20Ameera-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RM Therapeutics Pharmacology
spellingShingle	RM Therapeutics Pharmacology Shaheran, Anish Ameera Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
description	Edible bird nest (EBN) is one of the natural products that have high therapeutic potential and pharmacological properties. Therefore, this study aimed to determine the toxicity and antioxidants activity on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan (Tanah Merah, Tumpat and Jeli). The samples were extracted using three solvents which are distilled water, sodium hydroxide and hydrochloric acid. The toxicity of the EBN samples with different solvent of extraction were tested using MTT assay against cancerous (HeLa and MCF-7) and non-cancerous cell line (SVG p12). Minerals content in EBN was studied using inductively couple plasma mass spectrometry (ICP-MS) and the other toxicity test was brine shrimp lethality assay (BSLA) for LC50 values. The antioxidants activity was tested on EBN samples extracts using DPPH radical scavenging assay and Ferric reducing power assay (FRAP). EBN samples extracts showed higher inhibition activity against MCF- 7 cell line. Meanwhile, three samples of EBN extracts induced cell proliferation on SVG cell line as the IC50 value more than 100%. Minerals content in all EBN samples were below the maximum regulatory limit as suggested by FDA, WHO and SIRIM guidelines except sodium content that slightly higher than 2 ppm. The results showed no LC50 value was determine on EBN against brine shrimp. The antioxidants activity using DPPH and FRAP assay showed that NaOH extracts from three sample EBN had the lowest IC50 values and the highest reducing power. Thus, the results finding showed that the EBN samples extracts can be considered as cancer-preventive agents and are relatively safe to be consumed. Extracts of EBN samples can be considered asantioxidants agent. However, further validation have to be performed to understand the active compounds that poses safety, toxicity and antioxidants activity of EBN.
format	Thesis
qualification_level	Master's degree
author	Shaheran, Anish Ameera
author_facet	Shaheran, Anish Ameera
author_sort	Shaheran, Anish Ameera
title	Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
title_short	Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
title_full	Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
title_fullStr	Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
title_full_unstemmed	Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan
title_sort	determination of toxicity and antioxidants on white edible bird nest (aerodramus fuciphagus) from different district in kelantan
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/45815/1/Dr.%20Anish%20Ameera-24%20pages.pdf
_version_	1747821572981260288

Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan

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