The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7

Vernonia amygdalina (VA) is a broadly used local plant in Africa for both nutritional and therapeutic purposes. Nevertheless, different geographical origins, locality, climate conditions and time of harvesting could influence the biological activities of the plant. Albeit of the advancement in me...

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Main Author:	Jian, Chin Tze
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RD Surgery
Online Access:	http://eprints.usm.my/45852/1/Dr.%20Chin%20Tze%20Jian-24%20pages.pdf
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id	my-usm-ep.45852
record_format	uketd_dc
spelling	my-usm-ep.458522019-12-17T07:32:23Z The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7 2018-01 Jian, Chin Tze RD Surgery Vernonia amygdalina (VA) is a broadly used local plant in Africa for both nutritional and therapeutic purposes. Nevertheless, different geographical origins, locality, climate conditions and time of harvesting could influence the biological activities of the plant. Albeit of the advancement in medical field, the prevalence of breast cancer still increases globally and many data have shown that among all the different types of cancers, it is one of the cancers that contribute to the majority of the mortality. Moreover, the risk and detrimental side effects of chemotherapy are fast compensating its benefits. Therefore, this study focused on combination treatment of local VA and tamoxifen towards breast cancer cell line, MCF7 in an effort to reduce the nonselective effect on normal cells, NIH 3T3. The cytotoxicity activities of VA alone and combination with tamoxifen towards MCF7 and NIH 3T3 cell lines were examined using MTT assay. The IC50 values on MCF7 for VA (1.10 ± 1.78 mg/ml), tamoxifen (0.06 ± 0.04 mg/ml) and combination (0.09 ± 0.10 mg/ml) were obtained. VA was also found to be non-deleterious towards normal cells and had the IC50 values of NIH 3T3 raised in combination treatment (tamoxifen alone: 0.28 ± 0.22 mg/ml, combination: 3.69 ± 2.74 mg/ml). Antagonistic combination (CI>1) was also discovered between VA and tamoxifen. The combination treatment managed to have an anti-proliferative effect on MCF7 cells and reduced toxicity on normal cells.Nuclear staining by Hoechst 33258 suggested the occurrence of apoptosis based on the presence of bright fluorescence in the cells treated with combination treatment. In conclusion, VA exhibited potential selective anticancer properties in MCF7 cells by possibly inducing apoptotic cell death and it has a promising opportunity to be used clinically at the future. 2018-01 Thesis http://eprints.usm.my/45852/ http://eprints.usm.my/45852/1/Dr.%20Chin%20Tze%20Jian-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RD Surgery
spellingShingle	RD Surgery Jian, Chin Tze The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
description	Vernonia amygdalina (VA) is a broadly used local plant in Africa for both nutritional and therapeutic purposes. Nevertheless, different geographical origins, locality, climate conditions and time of harvesting could influence the biological activities of the plant. Albeit of the advancement in medical field, the prevalence of breast cancer still increases globally and many data have shown that among all the different types of cancers, it is one of the cancers that contribute to the majority of the mortality. Moreover, the risk and detrimental side effects of chemotherapy are fast compensating its benefits. Therefore, this study focused on combination treatment of local VA and tamoxifen towards breast cancer cell line, MCF7 in an effort to reduce the nonselective effect on normal cells, NIH 3T3. The cytotoxicity activities of VA alone and combination with tamoxifen towards MCF7 and NIH 3T3 cell lines were examined using MTT assay. The IC50 values on MCF7 for VA (1.10 ± 1.78 mg/ml), tamoxifen (0.06 ± 0.04 mg/ml) and combination (0.09 ± 0.10 mg/ml) were obtained. VA was also found to be non-deleterious towards normal cells and had the IC50 values of NIH 3T3 raised in combination treatment (tamoxifen alone: 0.28 ± 0.22 mg/ml, combination: 3.69 ± 2.74 mg/ml). Antagonistic combination (CI>1) was also discovered between VA and tamoxifen. The combination treatment managed to have an anti-proliferative effect on MCF7 cells and reduced toxicity on normal cells.Nuclear staining by Hoechst 33258 suggested the occurrence of apoptosis based on the presence of bright fluorescence in the cells treated with combination treatment. In conclusion, VA exhibited potential selective anticancer properties in MCF7 cells by possibly inducing apoptotic cell death and it has a promising opportunity to be used clinically at the future.
format	Thesis
qualification_level	Master's degree
author	Jian, Chin Tze
author_facet	Jian, Chin Tze
author_sort	Jian, Chin Tze
title	The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
title_short	The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
title_full	The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
title_fullStr	The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
title_full_unstemmed	The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7
title_sort	anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, mcf-7
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/45852/1/Dr.%20Chin%20Tze%20Jian-24%20pages.pdf
_version_	1747821580566659072

The anticancer effect of local vernonia amygdalina in combination with standard chemotherapy drug, tamoxifen on breast cancer cell line, MCF-7

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