mRNA Expression Of Bone Morphogenetic Protein 7 (bmp7) During Folliculogenesis In Zebrafish
The potential roles of bone morphogenetic protein 7 (bmp7) in ovary have drawn increasing attention. Present study aims to investigate the expression pattern of bmp7a and bmp7b in zebrafish. Adult female tissues were sacrificed for sampling of brain, eye, gill, heart, intestine, kidney, ovary, li...
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my-usm-ep.458882020-01-15T01:58:11Z mRNA Expression Of Bone Morphogenetic Protein 7 (bmp7) During Folliculogenesis In Zebrafish 2011-12 Lim, Phaik Siew QH1 Natural history (General - Including nature conservation, geographical distribution) The potential roles of bone morphogenetic protein 7 (bmp7) in ovary have drawn increasing attention. Present study aims to investigate the expression pattern of bmp7a and bmp7b in zebrafish. Adult female tissues were sacrificed for sampling of brain, eye, gill, heart, intestine, kidney, ovary, liver and swim bladder. For follicle compartments, follicle layers were mechanically separated from oocytes after cold-shock treatment. To verify analysis of bmp7a and bmp7b expression during different follicle stages, previtellogenic; early- and mid-vitellogenic mixed; full-grown immature at daytime, 0300 h, 0400 h; maturation (0500 h) and ovulation (0600 h) stage were isolated from dissected ovaries. Using semi-quantitative real-time PCR, bmp7a was found to be abundantly expressed in ovary whereas bmp7b was highly expressed in swim bladder, brain, eye, gill and ovary. Both molecules were predominantly expressed in the follicle layers rather than the oocyte. During folliculogenesis, bmp7a and bmp7b were expressed highly at previtellogenic stage. Thereafter, bmp7a remained rather constant across follicle developmental stages. In contrast, bmp7b expression progressively reduced to extremely low level at ovulation across the developing follicles. Overall, this finding indicated the possible importance of bmp7b as an autocrine/paracrine growth factor for follicular development at previtellogenic and, as repressor of oocyte maturation during folliculogenesis. 2011-12 Thesis http://eprints.usm.my/45888/ http://eprints.usm.my/45888/1/LIM%20PHAIK%20SIEW_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
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Universiti Sains Malaysia |
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USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
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QH1 Natural history (General - Including nature conservation geographical distribution) Lim, Phaik Siew mRNA Expression Of Bone Morphogenetic Protein 7 (bmp7) During Folliculogenesis In Zebrafish |
| description |
The potential roles of bone morphogenetic protein 7 (bmp7) in ovary have drawn
increasing attention. Present study aims to investigate the expression pattern of bmp7a and
bmp7b in zebrafish. Adult female tissues were sacrificed for sampling of brain, eye, gill,
heart, intestine, kidney, ovary, liver and swim bladder. For follicle compartments, follicle
layers were mechanically separated from oocytes after cold-shock treatment. To verify
analysis of bmp7a and bmp7b expression during different follicle stages, previtellogenic;
early- and mid-vitellogenic mixed; full-grown immature at daytime, 0300 h, 0400 h;
maturation (0500 h) and ovulation (0600 h) stage were isolated from dissected ovaries.
Using semi-quantitative real-time PCR, bmp7a was found to be abundantly expressed in
ovary whereas bmp7b was highly expressed in swim bladder, brain, eye, gill and ovary. Both
molecules were predominantly expressed in the follicle layers rather than the oocyte. During
folliculogenesis, bmp7a and bmp7b were expressed highly at previtellogenic stage.
Thereafter, bmp7a remained rather constant across follicle developmental stages. In contrast,
bmp7b expression progressively reduced to extremely low level at ovulation across the
developing follicles. Overall, this finding indicated the possible importance of bmp7b as an
autocrine/paracrine growth factor for follicular development at previtellogenic and, as
repressor of oocyte maturation during folliculogenesis. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Lim, Phaik Siew |
| author_facet |
Lim, Phaik Siew |
| author_sort |
Lim, Phaik Siew |
| title |
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish |
| title_short |
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish |
| title_full |
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish |
| title_fullStr |
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish |
| title_full_unstemmed |
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish |
| title_sort |
mrna expression of bone
morphogenetic protein 7 (bmp7) during
folliculogenesis in zebrafish |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2011 |
| url |
http://eprints.usm.my/45888/1/LIM%20PHAIK%20SIEW_HJ.pdf |
| _version_ |
1747821583787884544 |