Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques

Intestinal parasitic infections by helminths and protozoa are among the most prevalent infections and remain a major public health burden in underdeveloped countries. Most intestinal helminth infections cause abdominal pain accompanied by aneroxia, nausea and diarrhea while intestinal protozoa ca...

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Main Author:	Basuni, Madihah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/46047/1/MADIHAH%20BINTI%20BASUNI_HJ.pdf
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id	my-usm-ep.46047
record_format	uketd_dc
spelling	my-usm-ep.460472020-01-30T07:29:15Z Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques 2012-01 Basuni, Madihah R5-920 Medicine (General) Intestinal parasitic infections by helminths and protozoa are among the most prevalent infections and remain a major public health burden in underdeveloped countries. Most intestinal helminth infections cause abdominal pain accompanied by aneroxia, nausea and diarrhea while intestinal protozoa cause diarrheal diseases. Routine diagnostic methods for intestinal parasitic infections which rely heavily on microscopic detection are insensitive, and require well-trained microscopists to avoid misdiagnosis or overdiagnosis of the infection. These limitations have led to the development of highly sensitive DNA-based assays. PCR has been proven to be sensitive and specific for detection of enteric pathogens. Since conventional PCR is time consuming and prone to cross-contamination, it is desirable to develop a realtime PCR assay which is rapid and can provide quantitative and real-time information on the amplified products. In this study, a real-time multiplex PCR assay for the detection of closely related intestinal helminths namely Ascaris lumbricoides, Strongyloides stercoralis, Necator americanus and Ancylostoma duodenale and an assay for the detection of three closely related intestinal protozoa which comprised Entamoeba histolytica, Cryptosporidium parvum and Giardia lamblia were optimized and evaluated. 2012-01 Thesis http://eprints.usm.my/46047/ http://eprints.usm.my/46047/1/MADIHAH%20BINTI%20BASUNI_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Basuni, Madihah Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
description	Intestinal parasitic infections by helminths and protozoa are among the most prevalent infections and remain a major public health burden in underdeveloped countries. Most intestinal helminth infections cause abdominal pain accompanied by aneroxia, nausea and diarrhea while intestinal protozoa cause diarrheal diseases. Routine diagnostic methods for intestinal parasitic infections which rely heavily on microscopic detection are insensitive, and require well-trained microscopists to avoid misdiagnosis or overdiagnosis of the infection. These limitations have led to the development of highly sensitive DNA-based assays. PCR has been proven to be sensitive and specific for detection of enteric pathogens. Since conventional PCR is time consuming and prone to cross-contamination, it is desirable to develop a realtime PCR assay which is rapid and can provide quantitative and real-time information on the amplified products. In this study, a real-time multiplex PCR assay for the detection of closely related intestinal helminths namely Ascaris lumbricoides, Strongyloides stercoralis, Necator americanus and Ancylostoma duodenale and an assay for the detection of three closely related intestinal protozoa which comprised Entamoeba histolytica, Cryptosporidium parvum and Giardia lamblia were optimized and evaluated.
format	Thesis
qualification_level	Master's degree
author	Basuni, Madihah
author_facet	Basuni, Madihah
author_sort	Basuni, Madihah
title	Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
title_short	Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
title_full	Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
title_fullStr	Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
title_full_unstemmed	Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
title_sort	multiplex real-time pcr for the detection of pathogenic intestinal parasites and comparison with parasitological techniques
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2012
url	http://eprints.usm.my/46047/1/MADIHAH%20BINTI%20BASUNI_HJ.pdf
_version_	1747821599929663488

Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques

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