Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii

Toxoplasma gondii is an important pathogen in veterinary and human medicines. It is widely distributed throughout the world and has a broad range of hosts that includes warm-blooded animals as intermediate hosts and felid (cat) as the definitive host. Infection with this parasite is usually asymp...

Full description

Saved in:

Bibliographic Details
Main Author:	Rahumatullah, Anizah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/46048/1/Anizah_HJ.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.46048
record_format	uketd_dc
spelling	my-usm-ep.460482020-01-30T07:33:22Z Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii 2012-01 Rahumatullah, Anizah R5-920 Medicine (General) Toxoplasma gondii is an important pathogen in veterinary and human medicines. It is widely distributed throughout the world and has a broad range of hosts that includes warm-blooded animals as intermediate hosts and felid (cat) as the definitive host. Infection with this parasite is usually asymptomatic in healthy individuals but can cause morbidity and mortality in immunocompromised patients and in congenitally infected infants. Molecular biology is increasingly being used as a diagnostic method for diagnosis of toxoplasmosis, especially for specimens collected from amniotic fluid, cerebrospinal fluid, placenta tissue, blood and eye fluid. However, the existing PCRbased methods for the detection of Toxoplasma DNA suffer from variations in performance among the laboratories. Indeed, the use of PCR-based assay for diagnosis of Toxoplasma is still uncommon in Malaysia. The aim of this study is to develop rapid, specific and sensitive PCR-based assays to detect T. gondii DNA for diagnosis of toxoplasmosis. Three PCR-based assays namely conventional multiplex PCR, realtime multiplex PCR and thermostabilised PCR for the detection of T. gondii DNA were developed. All the primers and probes used were newly designed and optimized. All three PCR assays showed 100% specificity whereby no cross-reactivity with other organisms was observed. 2012-01 Thesis http://eprints.usm.my/46048/ http://eprints.usm.my/46048/1/Anizah_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Rahumatullah, Anizah Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
description	Toxoplasma gondii is an important pathogen in veterinary and human medicines. It is widely distributed throughout the world and has a broad range of hosts that includes warm-blooded animals as intermediate hosts and felid (cat) as the definitive host. Infection with this parasite is usually asymptomatic in healthy individuals but can cause morbidity and mortality in immunocompromised patients and in congenitally infected infants. Molecular biology is increasingly being used as a diagnostic method for diagnosis of toxoplasmosis, especially for specimens collected from amniotic fluid, cerebrospinal fluid, placenta tissue, blood and eye fluid. However, the existing PCRbased methods for the detection of Toxoplasma DNA suffer from variations in performance among the laboratories. Indeed, the use of PCR-based assay for diagnosis of Toxoplasma is still uncommon in Malaysia. The aim of this study is to develop rapid, specific and sensitive PCR-based assays to detect T. gondii DNA for diagnosis of toxoplasmosis. Three PCR-based assays namely conventional multiplex PCR, realtime multiplex PCR and thermostabilised PCR for the detection of T. gondii DNA were developed. All the primers and probes used were newly designed and optimized. All three PCR assays showed 100% specificity whereby no cross-reactivity with other organisms was observed.
format	Thesis
qualification_level	Master's degree
author	Rahumatullah, Anizah
author_facet	Rahumatullah, Anizah
author_sort	Rahumatullah, Anizah
title	Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
title_short	Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
title_full	Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
title_fullStr	Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
title_full_unstemmed	Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii
title_sort	development of conventional real-time and thermostabilised multiplex pcr for the detection of toxoplasma gondii
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2012
url	http://eprints.usm.my/46048/1/Anizah_HJ.pdf
_version_	1747821600175030272

Development Of Conventional Real-Time And Thermostabilised Multiplex PCR For The Detection Of Toxoplasma Gondii

Similar Items