The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish

Preliminary screening was done for 13 plants species belonging to member of nine families such as Senna spectabilis, Jacaranda filicifolia, Samanae saman, Tamarindus indica, Carica papaya, Andira inermis, Morinda elliptica, Coleus aromaticus benth, Citrus hystrix, Milletia pinnata, Cymbopogon nardus...

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Main Author: Ali, Azirah Akbar
Format: Thesis
Language:English
Published: 2014
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Online Access:http://eprints.usm.my/46052/1/Azirah%20Bt%20Akbar%20Ali24.pdf
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spelling my-usm-ep.460522020-01-31T01:34:12Z The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish 2014-02 Ali, Azirah Akbar QH1 Natural history (General - Including nature conservation, geographical distribution) Preliminary screening was done for 13 plants species belonging to member of nine families such as Senna spectabilis, Jacaranda filicifolia, Samanae saman, Tamarindus indica, Carica papaya, Andira inermis, Morinda elliptica, Coleus aromaticus benth, Citrus hystrix, Milletia pinnata, Cymbopogon nardus, Cymbopogon citratus and Polyalthia longifolia were screened to find an antibacterial activity against three common fish pathogens namely Aeromonas hydrophila, Staphylococcus xylosus and Streptococcus agalactiae. Minimum Inhibitory Concentration (MIC) showed, S. xylosus was inhibited by S. saman with lowest concentration at 0.625 mg/ml compared to T. indica and J. filicifolia; 2.5 mg/ml respectively. Tamarindus indica and J. filicifolia showed activity against all pathogens tested. Toxicity test of brine shrimp was conducted on three selected plants extract by using Artemia salina nauplii as a test sample and from the results, T. indica and J. filicifolia were highly toxic; and S. saman shows lower toxicity (>1.0 mg/ml); with LC50 value of 2.22 mg/ml. Scanning electron microscope (SEM) were used to observe on two pathogens; S. xylosus and S. agalactiae treated with S. saman leaf extracts and showed structural damage in the treated cells. In addition, phytochemical analysis was conducted to investigate the chemical constitution in S. saman leaf extract; and found presence of alkaloid, flavonoid, tannins and polyphenolic compounds. 2014-02 Thesis http://eprints.usm.my/46052/ http://eprints.usm.my/46052/1/Azirah%20Bt%20Akbar%20Ali24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Ali, Azirah Akbar
The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
description Preliminary screening was done for 13 plants species belonging to member of nine families such as Senna spectabilis, Jacaranda filicifolia, Samanae saman, Tamarindus indica, Carica papaya, Andira inermis, Morinda elliptica, Coleus aromaticus benth, Citrus hystrix, Milletia pinnata, Cymbopogon nardus, Cymbopogon citratus and Polyalthia longifolia were screened to find an antibacterial activity against three common fish pathogens namely Aeromonas hydrophila, Staphylococcus xylosus and Streptococcus agalactiae. Minimum Inhibitory Concentration (MIC) showed, S. xylosus was inhibited by S. saman with lowest concentration at 0.625 mg/ml compared to T. indica and J. filicifolia; 2.5 mg/ml respectively. Tamarindus indica and J. filicifolia showed activity against all pathogens tested. Toxicity test of brine shrimp was conducted on three selected plants extract by using Artemia salina nauplii as a test sample and from the results, T. indica and J. filicifolia were highly toxic; and S. saman shows lower toxicity (>1.0 mg/ml); with LC50 value of 2.22 mg/ml. Scanning electron microscope (SEM) were used to observe on two pathogens; S. xylosus and S. agalactiae treated with S. saman leaf extracts and showed structural damage in the treated cells. In addition, phytochemical analysis was conducted to investigate the chemical constitution in S. saman leaf extract; and found presence of alkaloid, flavonoid, tannins and polyphenolic compounds.
format Thesis
qualification_level Master's degree
author Ali, Azirah Akbar
author_facet Ali, Azirah Akbar
author_sort Ali, Azirah Akbar
title The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
title_short The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
title_full The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
title_fullStr The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
title_full_unstemmed The Efficacy Of Herbal Plants Extract Against Infection Of Aeromonas Hydrophila, Staphylococcus Xylosus And Streptococcus Agalactiae In Snakehead (Channa Striata) Fish
title_sort efficacy of herbal plants extract against infection of aeromonas hydrophila, staphylococcus xylosus and streptococcus agalactiae in snakehead (channa striata) fish
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2014
url http://eprints.usm.my/46052/1/Azirah%20Bt%20Akbar%20Ali24.pdf
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