Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos

Effective propagation of in vitro callus, shoot, plantlet and somatic embryo cultures of Eurycoma longifolia was achieved using micropropagation technique. In vitro shoots, somatic embryos and plantlet cultures were used for conducting histological and SEM analysis, chemotaxis assays and transformat...

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主要作者:	Balakrishnan, Bhavani
格式:	Thesis
語言:	English
出版:	2013
主題:	QH1 Natural history (General - Including nature conservation geographical distribution)
在線閱讀:	http://eprints.usm.my/46057/1/Bhavani%20Balakrishnan.pdf
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id	my-usm-ep.46057
record_format	uketd_dc
spelling	my-usm-ep.460572020-01-31T02:16:03Z Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos 2013-12 Balakrishnan, Bhavani QH1 Natural history (General - Including nature conservation, geographical distribution) Effective propagation of in vitro callus, shoot, plantlet and somatic embryo cultures of Eurycoma longifolia was achieved using micropropagation technique. In vitro shoots, somatic embryos and plantlet cultures were used for conducting histological and SEM analysis, chemotaxis assays and transformation with four different Agrobacterium rhizogenes disarmed strains. Histological analysis and SEM observations revealed that somatic embryos of Eurycoma longifolia share similar traits with zygotic embryos. Positive chemotactic responses of A. rhizogenes towards somatic embryo explants were detected using swarm plate assay. In vitro shoot and plantlet cultures were used to obtain leaves, stems and rachis explants for transformation. Hairy root-like structures were seen emerging from in vitro stem explants co-cultivated with Agrobacterium rhizogenes strain AR14. However, the number of explants expressing these hairy root-like structures was too low to be confirmed using GUS assay or PCR based detection of transgenes. Transformation optimisations with somatic embryo explants were conducted by analysing the effect of four different factors on the GUS positive frequency. It was ascertained that AR12 is the most virulent and suitable Agrobacterium rhizogenes strain for transformation of E. longifolia somatic embryos. 2013-12 Thesis http://eprints.usm.my/46057/ http://eprints.usm.my/46057/1/Bhavani%20Balakrishnan.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Balakrishnan, Bhavani Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
description	Effective propagation of in vitro callus, shoot, plantlet and somatic embryo cultures of Eurycoma longifolia was achieved using micropropagation technique. In vitro shoots, somatic embryos and plantlet cultures were used for conducting histological and SEM analysis, chemotaxis assays and transformation with four different Agrobacterium rhizogenes disarmed strains. Histological analysis and SEM observations revealed that somatic embryos of Eurycoma longifolia share similar traits with zygotic embryos. Positive chemotactic responses of A. rhizogenes towards somatic embryo explants were detected using swarm plate assay. In vitro shoot and plantlet cultures were used to obtain leaves, stems and rachis explants for transformation. Hairy root-like structures were seen emerging from in vitro stem explants co-cultivated with Agrobacterium rhizogenes strain AR14. However, the number of explants expressing these hairy root-like structures was too low to be confirmed using GUS assay or PCR based detection of transgenes. Transformation optimisations with somatic embryo explants were conducted by analysing the effect of four different factors on the GUS positive frequency. It was ascertained that AR12 is the most virulent and suitable Agrobacterium rhizogenes strain for transformation of E. longifolia somatic embryos.
format	Thesis
qualification_level	Master's degree
author	Balakrishnan, Bhavani
author_facet	Balakrishnan, Bhavani
author_sort	Balakrishnan, Bhavani
title	Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
title_short	Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
title_full	Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
title_fullStr	Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
title_full_unstemmed	Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos
title_sort	preliminary assessment of conditions influencing agrobacterium rhizogenes- mediated transformation of eurycoma longifolia jack. somatic embryos
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2013
url	http://eprints.usm.my/46057/1/Bhavani%20Balakrishnan.pdf
_version_	1747821602128527360

Preliminary Assessment Of Conditions Influencing Agrobacterium Rhizogenes- Mediated Transformation Of Eurycoma Longifolia Jack. Somatic Embryos

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