Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia

Mitochondrial cytochrome oxidase subunit I (COI) gene was utilised to assess the population genetics and phylogeographic relationships of the commercially important black scar oyster, C. iredalei among 11 populations throughout Peninsular Malaysia and Sabah. Overall, populations of C. iredalei demon...

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Main Author:	Abidin, Danial Hariz Zainal
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46084/1/Danial%20Hariz%20Bin%20Zainal%20Abidin24.pdf
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id	my-usm-ep.46084
record_format	uketd_dc
spelling	my-usm-ep.460842020-02-05T01:26:12Z Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia 2013-12 Abidin, Danial Hariz Zainal QH1-278.5 Natural history (General) Mitochondrial cytochrome oxidase subunit I (COI) gene was utilised to assess the population genetics and phylogeographic relationships of the commercially important black scar oyster, C. iredalei among 11 populations throughout Peninsular Malaysia and Sabah. Overall, populations of C. iredalei demonstrated low nucleotide diversity π (0.000 – 0.004) and low-to-high haplotype diversity h (0.000 – 0.795) levels. Genetic structuring was detected between the Peninsular Malaysia and Sabah populations as revealed by the FST and AMOVA analyses. However, the COI gene analyses showed minimal and non-significant (P>0.05) population differentiation within the Peninsular Malaysia and Sabah regions. Relative genetic homogeneity was reported within the coastal populations of Peninsular Malaysia and Sabah, respectively. These could be attributed to both high larval dispersal along the east and west coasts and human-driven spat translocation between the two coastlines due to C. iredalei cultivation practices. The neutrality and mismatch distribution analyses supported the hypothesis that C. iredalei had experienced a/several bottleneck event(s), followed by population expansion. The molecular information obtained from this study can be incorporated in a pragmatic aquaculture management strategy of wild broodstock and the hatchery lines of C. iredalei in Malaysia. A Next Generation Sequencing (NGS) approach, Ion Torrent technology was utilized in the development of novel microsatellite markers in C. iredalei. Although a total number of 2424 microsatellite motif repeats were identified, of which, 137 qualified for primer design in the present study. 2013-12 Thesis http://eprints.usm.my/46084/ http://eprints.usm.my/46084/1/Danial%20Hariz%20Bin%20Zainal%20Abidin24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Abidin, Danial Hariz Zainal Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
description	Mitochondrial cytochrome oxidase subunit I (COI) gene was utilised to assess the population genetics and phylogeographic relationships of the commercially important black scar oyster, C. iredalei among 11 populations throughout Peninsular Malaysia and Sabah. Overall, populations of C. iredalei demonstrated low nucleotide diversity π (0.000 – 0.004) and low-to-high haplotype diversity h (0.000 – 0.795) levels. Genetic structuring was detected between the Peninsular Malaysia and Sabah populations as revealed by the FST and AMOVA analyses. However, the COI gene analyses showed minimal and non-significant (P>0.05) population differentiation within the Peninsular Malaysia and Sabah regions. Relative genetic homogeneity was reported within the coastal populations of Peninsular Malaysia and Sabah, respectively. These could be attributed to both high larval dispersal along the east and west coasts and human-driven spat translocation between the two coastlines due to C. iredalei cultivation practices. The neutrality and mismatch distribution analyses supported the hypothesis that C. iredalei had experienced a/several bottleneck event(s), followed by population expansion. The molecular information obtained from this study can be incorporated in a pragmatic aquaculture management strategy of wild broodstock and the hatchery lines of C. iredalei in Malaysia. A Next Generation Sequencing (NGS) approach, Ion Torrent technology was utilized in the development of novel microsatellite markers in C. iredalei. Although a total number of 2424 microsatellite motif repeats were identified, of which, 137 qualified for primer design in the present study.
format	Thesis
qualification_level	Master's degree
author	Abidin, Danial Hariz Zainal
author_facet	Abidin, Danial Hariz Zainal
author_sort	Abidin, Danial Hariz Zainal
title	Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
title_short	Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
title_full	Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
title_fullStr	Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
title_full_unstemmed	Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia
title_sort	population structure based on mitochondrial cytochrome oxidase subunit i (coi) gene in the black scar oyster, crassostrea iredalei (faustino, 1932) in malaysia
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2013
url	http://eprints.usm.my/46084/1/Danial%20Hariz%20Bin%20Zainal%20Abidin24.pdf
_version_	1747821607489896448

Population Structure Based On Mitochondrial Cytochrome Oxidase Subunit I (Coi) Gene In The Black Scar Oyster, Crassostrea Iredalei (Faustino, 1932) In Malaysia

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