Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation

In the present study, attempt was made to solve the problems by cultivating Chlorella vulgaris using organic fertilizer (derived from compost) instead of depending on chemical fertilizer. Under the supplement of organic nutrients, it was found that Chlorella vulgaris grown favourably with 100 mL of...

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Main Author: Lam, Man Kee
Format: Thesis
Language:English
Published: 2014
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Online Access:http://eprints.usm.my/46127/1/Lam%20Man%20Kee24.pdf
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spelling my-usm-ep.461272020-02-07T07:23:41Z Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation 2014-02 Lam, Man Kee TP1-1185 Chemical technology In the present study, attempt was made to solve the problems by cultivating Chlorella vulgaris using organic fertilizer (derived from compost) instead of depending on chemical fertilizer. Under the supplement of organic nutrients, it was found that Chlorella vulgaris grown favourably with 100 mL of organic fertilizer medium (or corresponded to nitrate content of 26.67 mg/L), 24 hours of continuous illumination and pH of 5. About 0.50 g/L of biomass yield was attained after 12 days of cultivation. Increasing the CO2 concentration to the cultivation could accelerate the growth of Chlorella vulgaris, however, reducing the CO2 removal efficiency. The highest CO2 removal efficiency, 92.2 %, was achieved by using atmosphere air (0.03 % of CO2). By using Bligh and Dyer extraction solvents (methanol to chloroform volume ratio of 2:1), about 18 % of lipid can be extracted from the dried Chlorella vulgaris biomass. The lipid was mainly comprised of unsaturated fatty acids, such as C18:1, C18:2 and C18:3. Through transesterification reaction parametric study, about 95 % of fatty acid methyl ester (FAME) or biodiesel was attained under the following conditions: methanol to THF to lipid molar ratio of 60:15:1, H2SO4 concentration of 21 wt.%, temperature of 60 °C and reaction time of 3 hours. In addition, the carbohydrate left over in the lipid-extracted microalgae biomass residues was successfully recovered for maltodextrin production (co-product). 2014-02 Thesis http://eprints.usm.my/46127/ http://eprints.usm.my/46127/1/Lam%20Man%20Kee24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic TP1-1185 Chemical technology
spellingShingle TP1-1185 Chemical technology
Lam, Man Kee
Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
description In the present study, attempt was made to solve the problems by cultivating Chlorella vulgaris using organic fertilizer (derived from compost) instead of depending on chemical fertilizer. Under the supplement of organic nutrients, it was found that Chlorella vulgaris grown favourably with 100 mL of organic fertilizer medium (or corresponded to nitrate content of 26.67 mg/L), 24 hours of continuous illumination and pH of 5. About 0.50 g/L of biomass yield was attained after 12 days of cultivation. Increasing the CO2 concentration to the cultivation could accelerate the growth of Chlorella vulgaris, however, reducing the CO2 removal efficiency. The highest CO2 removal efficiency, 92.2 %, was achieved by using atmosphere air (0.03 % of CO2). By using Bligh and Dyer extraction solvents (methanol to chloroform volume ratio of 2:1), about 18 % of lipid can be extracted from the dried Chlorella vulgaris biomass. The lipid was mainly comprised of unsaturated fatty acids, such as C18:1, C18:2 and C18:3. Through transesterification reaction parametric study, about 95 % of fatty acid methyl ester (FAME) or biodiesel was attained under the following conditions: methanol to THF to lipid molar ratio of 60:15:1, H2SO4 concentration of 21 wt.%, temperature of 60 °C and reaction time of 3 hours. In addition, the carbohydrate left over in the lipid-extracted microalgae biomass residues was successfully recovered for maltodextrin production (co-product).
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lam, Man Kee
author_facet Lam, Man Kee
author_sort Lam, Man Kee
title Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
title_short Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
title_full Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
title_fullStr Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
title_full_unstemmed Cultivation Of Chlorella Vulgaris Using Organic Fertilizer As Nutrient Source For Biodiesel, Maltodextrin Production And Co2-Biomitigation
title_sort cultivation of chlorella vulgaris using organic fertilizer as nutrient source for biodiesel, maltodextrin production and co2-biomitigation
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Kejuruteraan Kimia
publishDate 2014
url http://eprints.usm.my/46127/1/Lam%20Man%20Kee24.pdf
_version_ 1747821615801958400