Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park

The unique ecosystems in Malaysia can be utilized as potential sources for isolating actinomycetes isolates that produce interesting bioactive secondary metabolites. This secondary metabolite exhibits a wide range of bioactivity including antitrypanosomal activity. The option of new lead compound is...

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Main Author:	Husin, Lili Sahira
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46129/1/Lili%20Sahira%20Binti%20Husin24.pdf
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id	my-usm-ep.46129
record_format	uketd_dc
spelling	my-usm-ep.461292020-02-07T07:32:33Z Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park 2014-01 Husin, Lili Sahira QH1-278.5 Natural history (General) The unique ecosystems in Malaysia can be utilized as potential sources for isolating actinomycetes isolates that produce interesting bioactive secondary metabolites. This secondary metabolite exhibits a wide range of bioactivity including antitrypanosomal activity. The option of new lead compound is needed for the disease treatment that caused by the trypanosome parasite since the existing drugs to cure are very limited due to drug resistance. In this study, a total of 83 actinomycetes isolates were successfully revived from cryovials stored at -80oC in FRIM Actinomycetes Culture Collection (FACC). The isolates were grown in three different fermentation media (M1, M2, and M3) and a total of 249 culture broth extracts obtained were evaluated for in vitro antitrypanosomal assay against Trypanosoma brucei brucei strain BS221 via primary, secondary and tertiary assays. Extracts that showed strong and moderate activities were evaluated for antitrypanosomal activity in tertiary assay and cytotoxicity to determine their selectivity index (SI) values. Among the active extracts that showed good selectivity toward the trypanosoma parasite, four extracts FACC-A032-M3, FACC-A026-M3, FACC-A048-M3 and FACC-A049-M3 produced strong antitrypanosomal activity (IC50 value < 1.56 g/ml) and high selectivity (SI  20). The potential isolates were further studied for their cultural, macromorphological, physiological and biochemical characteristics and spore chain type. Isolates FACC-A032 and FACC-A026 were tentatively identified as Streptomyces sp. and isolates FACC-A048 and FACC-A049 were categorized into the non-Streptomyces group. 2014-01 Thesis http://eprints.usm.my/46129/ http://eprints.usm.my/46129/1/Lili%20Sahira%20Binti%20Husin24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Husin, Lili Sahira Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
description	The unique ecosystems in Malaysia can be utilized as potential sources for isolating actinomycetes isolates that produce interesting bioactive secondary metabolites. This secondary metabolite exhibits a wide range of bioactivity including antitrypanosomal activity. The option of new lead compound is needed for the disease treatment that caused by the trypanosome parasite since the existing drugs to cure are very limited due to drug resistance. In this study, a total of 83 actinomycetes isolates were successfully revived from cryovials stored at -80oC in FRIM Actinomycetes Culture Collection (FACC). The isolates were grown in three different fermentation media (M1, M2, and M3) and a total of 249 culture broth extracts obtained were evaluated for in vitro antitrypanosomal assay against Trypanosoma brucei brucei strain BS221 via primary, secondary and tertiary assays. Extracts that showed strong and moderate activities were evaluated for antitrypanosomal activity in tertiary assay and cytotoxicity to determine their selectivity index (SI) values. Among the active extracts that showed good selectivity toward the trypanosoma parasite, four extracts FACC-A032-M3, FACC-A026-M3, FACC-A048-M3 and FACC-A049-M3 produced strong antitrypanosomal activity (IC50 value < 1.56 g/ml) and high selectivity (SI  20). The potential isolates were further studied for their cultural, macromorphological, physiological and biochemical characteristics and spore chain type. Isolates FACC-A032 and FACC-A026 were tentatively identified as Streptomyces sp. and isolates FACC-A048 and FACC-A049 were categorized into the non-Streptomyces group.
format	Thesis
qualification_level	Master's degree
author	Husin, Lili Sahira
author_facet	Husin, Lili Sahira
author_sort	Husin, Lili Sahira
title	Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
title_short	Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
title_full	Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
title_fullStr	Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
title_full_unstemmed	Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park
title_sort	antitrypanosomal secondary metabolites from soil actinomycetes isolated from penang national park
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2014
url	http://eprints.usm.my/46129/1/Lili%20Sahira%20Binti%20Husin24.pdf
_version_	1747821616287449088

Antitrypanosomal Secondary Metabolites From Soil Actinomycetes Isolated From Penang National Park

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