Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)

Drug abuse of amphetamine-type stimulants (ATS) has been considered as the most widespread problem around the world and also regarded as a threat to national security. This study is focused on stable carbon isotope analysis by isotope ratio mass spectrometry (IRMS) to develop and validate a method f...

Full description

Saved in:

Bibliographic Details
Main Author:	Obaid, Nayla Hilal M H
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/46182/1/Nayla%20Hilal%20M%20H%20Obaid24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.46182
record_format	uketd_dc
spelling	my-usm-ep.461822020-02-12T02:55:59Z Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms) 2013-11 Obaid, Nayla Hilal M H RS1-441 Pharmacy and materia medica Drug abuse of amphetamine-type stimulants (ATS) has been considered as the most widespread problem around the world and also regarded as a threat to national security. This study is focused on stable carbon isotope analysis by isotope ratio mass spectrometry (IRMS) to develop and validate a method for amphetamine and methamphetamine analysis. The ability to measure isotope distribution in natural abundance with high accuracy and precision has increased the application of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) for various applications in recent years. Urine Samples spiked with amphetamine and methamphetamine standards were extracted using liquid–liquid extraction and subsequently, reconstituted with the mobile phase for further purification and separation using High Performance Liquid Chromatography (HPLC). These fractions were collected and analysed using the GC/C/IRMS. GC/C/IRMS showed δ13C value of -24.72 ±0.6‰ and -28.90 ±0.15‰ for amphetamine methamphetamine, respectively. The solid samples of the same amphetamine and methamphetamine standards were also analysed to determine the δ13C of each standard by using the FLASH elemental analyzer isotope ratio mass spectrometry (EA/IRMS); these were then compared with the results obtained from the GC/C/IRMS. EA/IRMS showed comparable results with those obtained with GC/C/IRMS; amphetamine showed the δ13C value of -24.90 ±0.03‰, while methamphetamine showed δ13C result of -28.99 ±0.03‰. 2013-11 Thesis http://eprints.usm.my/46182/ http://eprints.usm.my/46182/1/Nayla%20Hilal%20M%20H%20Obaid24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Obaid, Nayla Hilal M H Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
description	Drug abuse of amphetamine-type stimulants (ATS) has been considered as the most widespread problem around the world and also regarded as a threat to national security. This study is focused on stable carbon isotope analysis by isotope ratio mass spectrometry (IRMS) to develop and validate a method for amphetamine and methamphetamine analysis. The ability to measure isotope distribution in natural abundance with high accuracy and precision has increased the application of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) for various applications in recent years. Urine Samples spiked with amphetamine and methamphetamine standards were extracted using liquid–liquid extraction and subsequently, reconstituted with the mobile phase for further purification and separation using High Performance Liquid Chromatography (HPLC). These fractions were collected and analysed using the GC/C/IRMS. GC/C/IRMS showed δ13C value of -24.72 ±0.6‰ and -28.90 ±0.15‰ for amphetamine methamphetamine, respectively. The solid samples of the same amphetamine and methamphetamine standards were also analysed to determine the δ13C of each standard by using the FLASH elemental analyzer isotope ratio mass spectrometry (EA/IRMS); these were then compared with the results obtained from the GC/C/IRMS. EA/IRMS showed comparable results with those obtained with GC/C/IRMS; amphetamine showed the δ13C value of -24.90 ±0.03‰, while methamphetamine showed δ13C result of -28.99 ±0.03‰.
format	Thesis
qualification_level	Master's degree
author	Obaid, Nayla Hilal M H
author_facet	Obaid, Nayla Hilal M H
author_sort	Obaid, Nayla Hilal M H
title	Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
title_short	Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
title_full	Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
title_fullStr	Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
title_full_unstemmed	Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)
title_sort	determination of delta 13c values of amphetamine and methamphetamine using gas chromatography/ combustion/ isotope ratio mass spectrometry (gc/c/irms)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2013
url	http://eprints.usm.my/46182/1/Nayla%20Hilal%20M%20H%20Obaid24.pdf
_version_	1747821627971731456

Determination Of Delta 13c Values Of Amphetamine And Methamphetamine Using Gas Chromatography/ Combustion/ Isotope Ratio Mass Spectrometry (Gc/C/Irms)

Similar Items