A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein

Burkholderia pseudomallei is the causative agent of melioidosis. From the whole genomic comparison by using B. pseudomallei K96243 as reference genome, a total 48 genes were found specifically and common in B. pseudomallei strains. Amongst, the hypothetical gene, BPSL3393, was deduced to contain the...

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Main Author:	Ooi, Gim Luan
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46193/1/Ooi%20Gim%20Luan24.pdf
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id	my-usm-ep.46193
record_format	uketd_dc
spelling	my-usm-ep.461932020-02-13T02:34:53Z A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein 2014-01 Ooi, Gim Luan QH1-278.5 Natural history (General) Burkholderia pseudomallei is the causative agent of melioidosis. From the whole genomic comparison by using B. pseudomallei K96243 as reference genome, a total 48 genes were found specifically and common in B. pseudomallei strains. Amongst, the hypothetical gene, BPSL3393, was deduced to contain the CoA-binding motif based on its sequence analysis. However, this gene is still remaining as a putative gene where it is still functionally uncharacterized. The objective of this study is to elucidate the biological function of gene BPSL3393 in B. pseudomallei K96243. An unmarked deletion mutant of gene BPSL3393 was constructed by using pDM4 suicidal vector. This vector employed catR, chloramphenicol resistant gene as the selection marker and sacB, levansucrase gene as the counter-selection marker. The biochemical profiles of ΔBPSL3393 mutant and wild type strains were determined by using th Biolog GN2 MicroPlateTM system. The ΔBPSL3393 mutant showed significant reduction in 2-aminoethanol utilization as compared to wild type. Apart from this, the ΔBPSL3393 mutant also has shown some physiological difference as compared to wild type. The ΔBPSL3393 mutant aggregated at the exponential phase in M9 minimal media whereas the wild type did not. Thus, further study is needed to characterize this ΔBPSL3393 mutant and to uncover its biological role. 2014-01 Thesis http://eprints.usm.my/46193/ http://eprints.usm.my/46193/1/Ooi%20Gim%20Luan24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Ooi, Gim Luan A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
description	Burkholderia pseudomallei is the causative agent of melioidosis. From the whole genomic comparison by using B. pseudomallei K96243 as reference genome, a total 48 genes were found specifically and common in B. pseudomallei strains. Amongst, the hypothetical gene, BPSL3393, was deduced to contain the CoA-binding motif based on its sequence analysis. However, this gene is still remaining as a putative gene where it is still functionally uncharacterized. The objective of this study is to elucidate the biological function of gene BPSL3393 in B. pseudomallei K96243. An unmarked deletion mutant of gene BPSL3393 was constructed by using pDM4 suicidal vector. This vector employed catR, chloramphenicol resistant gene as the selection marker and sacB, levansucrase gene as the counter-selection marker. The biochemical profiles of ΔBPSL3393 mutant and wild type strains were determined by using th Biolog GN2 MicroPlateTM system. The ΔBPSL3393 mutant showed significant reduction in 2-aminoethanol utilization as compared to wild type. Apart from this, the ΔBPSL3393 mutant also has shown some physiological difference as compared to wild type. The ΔBPSL3393 mutant aggregated at the exponential phase in M9 minimal media whereas the wild type did not. Thus, further study is needed to characterize this ΔBPSL3393 mutant and to uncover its biological role.
format	Thesis
qualification_level	Master's degree
author	Ooi, Gim Luan
author_facet	Ooi, Gim Luan
author_sort	Ooi, Gim Luan
title	A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
title_short	A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
title_full	A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
title_fullStr	A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
title_full_unstemmed	A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
title_sort	study of burkholderia pseudomallei k96243 hypothetical bpsl3393 gene product as a putative coa-binding protein
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2014
url	http://eprints.usm.my/46193/1/Ooi%20Gim%20Luan24.pdf
_version_	1747821630176886784

A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein

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