Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification

Isoamyl acetate is an important substance which is used in various industries including flavour and fragrance industry. The production of this compound using enzymes had attracted many attentions due to its advantages of moderate operating parameters, limited side reactions and easy product recov...

Full description

Saved in:
Bibliographic Details
Main Author: Hashim, Nur Hamizah Abdul Ghani @
Format: Thesis
Language:English
Published: 2011
Subjects:
Online Access:http://eprints.usm.my/46247/1/NUR%20HAMIZAH%20BINTI%20ABDUL%20GHANI%20%40%20HASHIM_HJ.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.46247
record_format uketd_dc
spelling my-usm-ep.462472020-02-18T01:11:36Z Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification 2011-06 Hashim, Nur Hamizah Abdul Ghani @ TN1-997 Mining engineering. Metallurgy Isoamyl acetate is an important substance which is used in various industries including flavour and fragrance industry. The production of this compound using enzymes had attracted many attentions due to its advantages of moderate operating parameters, limited side reactions and easy product recovery. In enzyme catalyzed esterification, acetic acid and isoamyl alcohol are used as substrates to the Candida antarctica immobilized lipase to produce isoamyl acetate. In direct esterification reaction, the ester synthesis is affected by excess water which can change the thermodynamic balance of reaction towards hydrolysis. On the other hand, during the initial stage of the reaction, water activates the enzymes and increases enzyme activity thus increases the reaction rates. Therefore, the amount of water content has been a critical parameter in enzyme catalyzed esterification. Beside this, few parameters considered to affect the process were temperature, enzyme loading, concentration of substrates and reaction time. In order to understand the process and the relationship of these selected parameters, process optimization and model development are essential. The processes optimizations were done via “one-factor-ata- time” and response surface methodology (RSM). The optimum conditions obtained from “one-factor-at-a-time” were compared with RSM and the results were in range. The coefficient of determination, R2 of the RSM model was 0.9448. 2011-06 Thesis http://eprints.usm.my/46247/ http://eprints.usm.my/46247/1/NUR%20HAMIZAH%20BINTI%20ABDUL%20GHANI%20%40%20HASHIM_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Bahan & Sumber Mineral
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic TN1-997 Mining engineering
Metallurgy
spellingShingle TN1-997 Mining engineering
Metallurgy
Hashim, Nur Hamizah Abdul Ghani @
Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
description Isoamyl acetate is an important substance which is used in various industries including flavour and fragrance industry. The production of this compound using enzymes had attracted many attentions due to its advantages of moderate operating parameters, limited side reactions and easy product recovery. In enzyme catalyzed esterification, acetic acid and isoamyl alcohol are used as substrates to the Candida antarctica immobilized lipase to produce isoamyl acetate. In direct esterification reaction, the ester synthesis is affected by excess water which can change the thermodynamic balance of reaction towards hydrolysis. On the other hand, during the initial stage of the reaction, water activates the enzymes and increases enzyme activity thus increases the reaction rates. Therefore, the amount of water content has been a critical parameter in enzyme catalyzed esterification. Beside this, few parameters considered to affect the process were temperature, enzyme loading, concentration of substrates and reaction time. In order to understand the process and the relationship of these selected parameters, process optimization and model development are essential. The processes optimizations were done via “one-factor-ata- time” and response surface methodology (RSM). The optimum conditions obtained from “one-factor-at-a-time” were compared with RSM and the results were in range. The coefficient of determination, R2 of the RSM model was 0.9448.
format Thesis
qualification_level Master's degree
author Hashim, Nur Hamizah Abdul Ghani @
author_facet Hashim, Nur Hamizah Abdul Ghani @
author_sort Hashim, Nur Hamizah Abdul Ghani @
title Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
title_short Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
title_full Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
title_fullStr Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
title_full_unstemmed Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification
title_sort modeling and optimization of isoamyl acetate production in enzyme catalyzed esterification
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Kejuruteraan Bahan & Sumber Mineral
publishDate 2011
url http://eprints.usm.my/46247/1/NUR%20HAMIZAH%20BINTI%20ABDUL%20GHANI%20%40%20HASHIM_HJ.pdf
_version_ 1747821636546985984