The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat

This study investigated whether the α1-adrenoceptor responsiveness of the renal vasculature was altered in a metabolic syndrome state due to high-fructose feeding. Male Sprague-Dawley rats were fed for 8 weeks with 20% fructose in the drinking water (F), while their controls received tap water (C) t...

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Main Author:	Abdulla, Mohammed H.
Format:	Thesis
Language:	English
Published:	2012
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/46249/1/Mohammed%20H.%20Abdulla_HJ.pdf
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id	my-usm-ep.46249
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spelling	my-usm-ep.462492020-02-18T01:57:24Z The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat 2012-02 Abdulla, Mohammed H. RS1-441 Pharmacy and materia medica This study investigated whether the α1-adrenoceptor responsiveness of the renal vasculature was altered in a metabolic syndrome state due to high-fructose feeding. Male Sprague-Dawley rats were fed for 8 weeks with 20% fructose in the drinking water (F), while their controls received tap water (C) to drink ad libitum. Metabolic, functional and haemodynamic parameters were assessed weekly and at the end of the study. In another set of rats, F received either carvedilol (FCV) or losartan (FL) at (10mg/kg/day po) for 3 weeks starting from week 5 of the experiment. Another group of rats received tempol, a superoxide dismutase mimetic (FT) at (1 mmol/L) with 20% fructose in drinking water for 8 weeks. At the end of the treatment period, an intravenous insulin glucose tolerance test was performed to assess insulin sensitivity. Moreover, rats were pentobarbitone anaesthetized and the reductions in renal cortical blood flow induced by intrarenal administration of noradrenaline (NA), phenylephrine (PE), methoxamine (ME) and angiotensin II (Ang II) were determined in the presence and absence of 5-methylurapidil (5-MU), chloroethylclonidine (CEC) or BMY 7378. Data, mean±SEM were subjected to ANOVA with significance at P<0.05. At the end of the 8 weeks, F had higher systemic blood pressure, plasma glucose, triglycerides and insulin levels but significantly lower absolute and fractional sodium and potassium excretion as compared to C. The F rats expressed reduced (P<0.05) renal vascular responses to NA, PE, ME and Ang II compared to C. The response to Ang II was significantly attenuated by 5-MU, CEC and BMY 7378, and also following carvedilol, losartan or tempol treatments in the F and C rats. 2012-02 Thesis http://eprints.usm.my/46249/ http://eprints.usm.my/46249/1/Mohammed%20H.%20Abdulla_HJ.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Abdulla, Mohammed H. The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
description	This study investigated whether the α1-adrenoceptor responsiveness of the renal vasculature was altered in a metabolic syndrome state due to high-fructose feeding. Male Sprague-Dawley rats were fed for 8 weeks with 20% fructose in the drinking water (F), while their controls received tap water (C) to drink ad libitum. Metabolic, functional and haemodynamic parameters were assessed weekly and at the end of the study. In another set of rats, F received either carvedilol (FCV) or losartan (FL) at (10mg/kg/day po) for 3 weeks starting from week 5 of the experiment. Another group of rats received tempol, a superoxide dismutase mimetic (FT) at (1 mmol/L) with 20% fructose in drinking water for 8 weeks. At the end of the treatment period, an intravenous insulin glucose tolerance test was performed to assess insulin sensitivity. Moreover, rats were pentobarbitone anaesthetized and the reductions in renal cortical blood flow induced by intrarenal administration of noradrenaline (NA), phenylephrine (PE), methoxamine (ME) and angiotensin II (Ang II) were determined in the presence and absence of 5-methylurapidil (5-MU), chloroethylclonidine (CEC) or BMY 7378. Data, mean±SEM were subjected to ANOVA with significance at P<0.05. At the end of the 8 weeks, F had higher systemic blood pressure, plasma glucose, triglycerides and insulin levels but significantly lower absolute and fractional sodium and potassium excretion as compared to C. The F rats expressed reduced (P<0.05) renal vascular responses to NA, PE, ME and Ang II compared to C. The response to Ang II was significantly attenuated by 5-MU, CEC and BMY 7378, and also following carvedilol, losartan or tempol treatments in the F and C rats.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Abdulla, Mohammed H.
author_facet	Abdulla, Mohammed H.
author_sort	Abdulla, Mohammed H.
title	The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
title_short	The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
title_full	The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
title_fullStr	The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
title_full_unstemmed	The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat
title_sort	effects of fructose-induced metabolic syndrome on renal haemodynamic and excretory function in rat
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2012
url	http://eprints.usm.my/46249/1/Mohammed%20H.%20Abdulla_HJ.pdf
_version_	1747821637034573824

The Effects Of Fructose-Induced Metabolic Syndrome On Renal Haemodynamic And Excretory Function In Rat

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