Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)

Red-fleshed dragon fruit (Hylocereus polyrhizus) is regarded as a health fruit crop and can bring high economic returns to Malaysia. The cultivation areas of this crop is increasing yearly and is now widely cultivated in Malaysia. However, H. polyrhizus is vulnerable towards multiple diseases caused...

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Main Author:	Vijaya, Suzianti Iskandar
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46259/1/Suzianti%20Binti%20Iskandar%20Vijaya24.pdf
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id	my-usm-ep.46259
record_format	uketd_dc
spelling	my-usm-ep.462592020-02-19T03:40:50Z Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus) 2014-01 Vijaya, Suzianti Iskandar QH1-278.5 Natural history (General) Red-fleshed dragon fruit (Hylocereus polyrhizus) is regarded as a health fruit crop and can bring high economic returns to Malaysia. The cultivation areas of this crop is increasing yearly and is now widely cultivated in Malaysia. However, H. polyrhizus is vulnerable towards multiple diseases caused by fungi, bacteria and virus, which is the major problem for H. polyrhizus cultivation in Malaysia. One of the important diseases caused by fungi infecting H. polyrhizus is anthracnose caused by Colletotrichum species. Thus, the main objectives of this study were to isolate, identify and characterize the Colletotrichum species associated with anthracnose of H. polyrhizus. The analyses were based on morphological characteristics, DNA sequencing of Internal Transcribed Spacer regions (ITS+5.8S) and β-tubulin gene, the genetic variation among the Colletotrichum isolates using phylogenetic analysis of ITS+5.8S and β-tubulin sequences and pathogenicity test. A total of 73 isolates of Colletotrichum were recovered from diseased H. polyrhizus stems from six states (Johor, Kedah, Negeri Sembilan, Pahang, Pulau Pinang and Selangor) in Peninsular Malaysia. Based on morphological characteristics, 63 isolates were identified as C. gloeosporioides and 10 isolates were identified as C. truncatum. Basic Local Alignment Search Tool (BLAST) similarity search of ITS+5.8S and ß-tubulin sequences confirmed the identity of morphologically identified C. gloeosporioides (63 isolates) and C. truncatum (10 isolates). Based on phylogenetic analysis, C. gloeosporioides and C. truncatum isolates were divided into two separate groups. 2014-01 Thesis http://eprints.usm.my/46259/ http://eprints.usm.my/46259/1/Suzianti%20Binti%20Iskandar%20Vijaya24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Vijaya, Suzianti Iskandar Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
description	Red-fleshed dragon fruit (Hylocereus polyrhizus) is regarded as a health fruit crop and can bring high economic returns to Malaysia. The cultivation areas of this crop is increasing yearly and is now widely cultivated in Malaysia. However, H. polyrhizus is vulnerable towards multiple diseases caused by fungi, bacteria and virus, which is the major problem for H. polyrhizus cultivation in Malaysia. One of the important diseases caused by fungi infecting H. polyrhizus is anthracnose caused by Colletotrichum species. Thus, the main objectives of this study were to isolate, identify and characterize the Colletotrichum species associated with anthracnose of H. polyrhizus. The analyses were based on morphological characteristics, DNA sequencing of Internal Transcribed Spacer regions (ITS+5.8S) and β-tubulin gene, the genetic variation among the Colletotrichum isolates using phylogenetic analysis of ITS+5.8S and β-tubulin sequences and pathogenicity test. A total of 73 isolates of Colletotrichum were recovered from diseased H. polyrhizus stems from six states (Johor, Kedah, Negeri Sembilan, Pahang, Pulau Pinang and Selangor) in Peninsular Malaysia. Based on morphological characteristics, 63 isolates were identified as C. gloeosporioides and 10 isolates were identified as C. truncatum. Basic Local Alignment Search Tool (BLAST) similarity search of ITS+5.8S and ß-tubulin sequences confirmed the identity of morphologically identified C. gloeosporioides (63 isolates) and C. truncatum (10 isolates). Based on phylogenetic analysis, C. gloeosporioides and C. truncatum isolates were divided into two separate groups.
format	Thesis
qualification_level	Master's degree
author	Vijaya, Suzianti Iskandar
author_facet	Vijaya, Suzianti Iskandar
author_sort	Vijaya, Suzianti Iskandar
title	Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
title_short	Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
title_full	Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
title_fullStr	Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
title_full_unstemmed	Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)
title_sort	morphological and molecular characterization of colletotrichum species isolated from anthracnose of red-fleshed dragon fruit (hylocereus polyrhizus)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2014
url	http://eprints.usm.my/46259/1/Suzianti%20Binti%20Iskandar%20Vijaya24.pdf
_version_	1747821638746898432

Morphological And Molecular Characterization Of Colletotrichum Species Isolated From Anthracnose Of Red-Fleshed Dragon Fruit (Hylocereus Polyrhizus)

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