Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood

Wood-degrading fungi are serious threat to rubberwood. Chemical preservatives commonly used in rubberwood preserving industry become awareness as it give impact to health and environmental problem. Biological control may be used as an alternative method to solve this problem. In this study, twelve l...

Full description

Saved in:

Bibliographic Details
Main Author:	Teoh, Yi Peng @ Teng Yi Peng
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/46268/1/Teoh%20Yi%20Peng24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.46268
record_format	uketd_dc
spelling	my-usm-ep.462682020-02-19T06:47:14Z Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood 2014-02 Teoh, Yi Peng @ Teng Yi Peng TP1-1185 Chemical technology Wood-degrading fungi are serious threat to rubberwood. Chemical preservatives commonly used in rubberwood preserving industry become awareness as it give impact to health and environmental problem. Biological control may be used as an alternative method to solve this problem. In this study, twelve locally isolated wild strains of wood-degrading fungi were screened for their capability to produce antifungal agent. Results showed that methanol extract of Schizophyllum commune biomass provided the highest antifungal activity among the tested fungi with minimum inhibitory concentration (MIC) ranging from 0.1 to 5.0 μg/μl. Chemical compound presence in the biomass extract was analyzed via gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatograph (HPLC). It was found that 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), a flavonoid fraction, was presence in the biomass extract. Optimization strategy based on one-factor-at-a-time (OFTA) method and statistical tool were employed to optimize the growth of S. commune in shake flask culture. Based on Plackett-Burman design (PBD), the variables such as yeast extract, glucose and MgSO4·7H2O significantly affected the fungus growth. The optimal values of these process variables were determined using Response Surface Methodology (RSM) coupled with Box-Behnken design (BBD). For the extraction of antifungal agent, the optimum conditions were 70.75% (v/v) methanol, 29 °C, and 145 rpm. All optimization studies were verified and the experimental data fitted well to the selected models with error percentage less than 1%. 2014-02 Thesis http://eprints.usm.my/46268/ http://eprints.usm.my/46268/1/Teoh%20Yi%20Peng24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Teoh, Yi Peng @ Teng Yi Peng Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
description	Wood-degrading fungi are serious threat to rubberwood. Chemical preservatives commonly used in rubberwood preserving industry become awareness as it give impact to health and environmental problem. Biological control may be used as an alternative method to solve this problem. In this study, twelve locally isolated wild strains of wood-degrading fungi were screened for their capability to produce antifungal agent. Results showed that methanol extract of Schizophyllum commune biomass provided the highest antifungal activity among the tested fungi with minimum inhibitory concentration (MIC) ranging from 0.1 to 5.0 μg/μl. Chemical compound presence in the biomass extract was analyzed via gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatograph (HPLC). It was found that 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP), a flavonoid fraction, was presence in the biomass extract. Optimization strategy based on one-factor-at-a-time (OFTA) method and statistical tool were employed to optimize the growth of S. commune in shake flask culture. Based on Plackett-Burman design (PBD), the variables such as yeast extract, glucose and MgSO4·7H2O significantly affected the fungus growth. The optimal values of these process variables were determined using Response Surface Methodology (RSM) coupled with Box-Behnken design (BBD). For the extraction of antifungal agent, the optimum conditions were 70.75% (v/v) methanol, 29 °C, and 145 rpm. All optimization studies were verified and the experimental data fitted well to the selected models with error percentage less than 1%.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Teoh, Yi Peng @ Teng Yi Peng
author_facet	Teoh, Yi Peng @ Teng Yi Peng
author_sort	Teoh, Yi Peng @ Teng Yi Peng
title	Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
title_short	Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
title_full	Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
title_fullStr	Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
title_full_unstemmed	Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood
title_sort	production of anti-fungal agent by schizophyllum commune against wood-degrading fungi of rubberwood
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2014
url	http://eprints.usm.my/46268/1/Teoh%20Yi%20Peng24.pdf
_version_	1747821640957296640

Production Of Anti-Fungal Agent By Schizophyllum Commune Against Wood-Degrading Fungi Of Rubberwood

Similar Items