Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks

Pectin-like polysaccharides were extracted from cocoa pod husk. The aim of this study was to develop a sustainable enzyme-assisted extraction approach in order to improve the extraction yield. Several extraction conditions (i.e. type of enzyme, concentration of enzyme, pH and temperature) were inves...

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Main Author:	Ahmad, Erna
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46277/1/Erna%20Binti%20Ahmad24.pdf
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id	my-usm-ep.46277
record_format	uketd_dc
spelling	my-usm-ep.462772020-02-20T02:01:08Z Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks 2014-02 Ahmad, Erna QH1-278.5 Natural history (General) Pectin-like polysaccharides were extracted from cocoa pod husk. The aim of this study was to develop a sustainable enzyme-assisted extraction approach in order to improve the extraction yield. Several extraction conditions (i.e. type of enzyme, concentration of enzyme, pH and temperature) were investigated followed by characterization of the extracted pectin-like polysaccharide based on physico-chemical and antioxidant properties. The results showed that high extraction yield of up to 41.5% (w/w) with the content of galacturonic acid ranged from 5.14 to 20.91% (w/w) and polysaccharide contents ranged from 6.12 to 61.18% were obtained. Different degrees of esterification (35 to 58%) were observed in these pectin-like polysaccharides. Other than that, the extracted pectin-like polysaccharide was found to possess high oil holding capacity ranging from 6.26 to 29.57 g oil/g extract. These extracts were found to have an ability to form gels and exhibited non-Newtonian shear-thinning behavior. Also, it was found that the extracts contain phenolic component with content ranging of 7.90 to 47.29 μg GAE/mg, percentages of 1,1-diphenyl-2-picryhydrazyl (DPPH) from 0.95 to 51.93% and FRAP values ranged from 0.43 to 3.07 μmol/mg extract. 2014-02 Thesis http://eprints.usm.my/46277/ http://eprints.usm.my/46277/1/Erna%20Binti%20Ahmad24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Ahmad, Erna Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
description	Pectin-like polysaccharides were extracted from cocoa pod husk. The aim of this study was to develop a sustainable enzyme-assisted extraction approach in order to improve the extraction yield. Several extraction conditions (i.e. type of enzyme, concentration of enzyme, pH and temperature) were investigated followed by characterization of the extracted pectin-like polysaccharide based on physico-chemical and antioxidant properties. The results showed that high extraction yield of up to 41.5% (w/w) with the content of galacturonic acid ranged from 5.14 to 20.91% (w/w) and polysaccharide contents ranged from 6.12 to 61.18% were obtained. Different degrees of esterification (35 to 58%) were observed in these pectin-like polysaccharides. Other than that, the extracted pectin-like polysaccharide was found to possess high oil holding capacity ranging from 6.26 to 29.57 g oil/g extract. These extracts were found to have an ability to form gels and exhibited non-Newtonian shear-thinning behavior. Also, it was found that the extracts contain phenolic component with content ranging of 7.90 to 47.29 μg GAE/mg, percentages of 1,1-diphenyl-2-picryhydrazyl (DPPH) from 0.95 to 51.93% and FRAP values ranged from 0.43 to 3.07 μmol/mg extract.
format	Thesis
qualification_level	Master's degree
author	Ahmad, Erna
author_facet	Ahmad, Erna
author_sort	Ahmad, Erna
title	Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
title_short	Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
title_full	Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
title_fullStr	Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
title_full_unstemmed	Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks
title_sort	enzyme-assisted extraction and physico-chemical characterization of pectin-like polysaccharides from theobroma cocoa pod husks
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2014
url	http://eprints.usm.my/46277/1/Erna%20Binti%20Ahmad24.pdf
_version_	1747821642670669824

Enzyme-Assisted Extraction And Physico-Chemical Characterization Of Pectin-Like Polysaccharides From Theobroma Cocoa Pod Husks

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