Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer...

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Main Author:	Ramachandran, Hema
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf
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id	my-usm-ep.46279
record_format	uketd_dc
spelling	my-usm-ep.462792020-02-20T02:15:30Z Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 2013-11 Ramachandran, Hema QH1-278.5 Natural history (General) Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer composition, (ii) explore the ability of the bacterium to produce P(3HB-co-4HB) using various inexpensive and renewable carbon sources, (iii) optimize the P(3HB-co-4HB) copolymer production using glycerine pitch through shake-flask fermentation using response surface methodology (RSM) and (iv) evaluate the material characteristics of the P(3HB-co-4HB) copolymers with various 4HB monomer compositions. In this study, a novel yellow-pigmented bacterium which exhibited ability of producing P(3HB-co-4HB) copolymer was successfully isolated from Perak, Malaysia and designated as Cupriavidus sp. USMAHM13. Based on the phenotypic and genotypic analyses, it could be suggested that Cupriavidus sp. USMAHM13 represents a novel species within the genus Cupriavidus. Preliminary screening of carbon sources for biosynthesis of P(3HB-co-4HB) copolymer by Cupriavidus sp. USMAHM13 revealed that high 4HB monomer composition (43 mol%) with cell dry weight and PHA content of 6.0 g/l and 49 wt%, respectively was achieved through combination of glycerine pitch (5 g/l) and 1,4-butanediol (5 g/l) via one-stage cultivation. 2013-11 Thesis http://eprints.usm.my/46279/ http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Ramachandran, Hema Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
description	Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer composition, (ii) explore the ability of the bacterium to produce P(3HB-co-4HB) using various inexpensive and renewable carbon sources, (iii) optimize the P(3HB-co-4HB) copolymer production using glycerine pitch through shake-flask fermentation using response surface methodology (RSM) and (iv) evaluate the material characteristics of the P(3HB-co-4HB) copolymers with various 4HB monomer compositions. In this study, a novel yellow-pigmented bacterium which exhibited ability of producing P(3HB-co-4HB) copolymer was successfully isolated from Perak, Malaysia and designated as Cupriavidus sp. USMAHM13. Based on the phenotypic and genotypic analyses, it could be suggested that Cupriavidus sp. USMAHM13 represents a novel species within the genus Cupriavidus. Preliminary screening of carbon sources for biosynthesis of P(3HB-co-4HB) copolymer by Cupriavidus sp. USMAHM13 revealed that high 4HB monomer composition (43 mol%) with cell dry weight and PHA content of 6.0 g/l and 49 wt%, respectively was achieved through combination of glycerine pitch (5 g/l) and 1,4-butanediol (5 g/l) via one-stage cultivation.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ramachandran, Hema
author_facet	Ramachandran, Hema
author_sort	Ramachandran, Hema
title	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
title_short	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
title_full	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
title_fullStr	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
title_full_unstemmed	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
title_sort	biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer from cupriavidus sp. usmahm13
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2013
url	http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf
_version_	1747821643162451968

Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13

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