Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis

Controlling sizes of nanomaterials are attracting a great deal of attention because of their unit properties for achieving specific processes especially in biological and medical applications. In this context, Ag which possesses good antimicrobial effects is of interest. A simple and effective appro...

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Main Author:	Chan, Yen San
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/46389/1/Chan%20Yen%20San24.pdf
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spelling	my-usm-ep.463892020-02-28T07:29:49Z Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis 2014-02 Chan, Yen San TP1-1185 Chemical technology Controlling sizes of nanomaterials are attracting a great deal of attention because of their unit properties for achieving specific processes especially in biological and medical applications. In this context, Ag which possesses good antimicrobial effects is of interest. A simple and effective approach for the synthesis of Ag nanoparticles by reduction mechanism of proteins synthesized by several species of white rot fungi was studied. Pycnoporus sanguineus was found to produce smallest Ag nanoparticles with degree of monodispersity. A Box-Behnken design was employed to optimize a fermentation medium for the production of protein by the selected fungi. The four variables involved in the study were glucose, malt extract, yeast extract and pH. The optimized medium contained 8.39 gL-1, 18.34 gL-1, 14.71 gL-1 of yeast extract, glucose and malt extract respectively with initial pH 6.66; and produced the maximum protein at 0.858 gL-1. Kinetic growth of the fungi, glucose consumption and product formation of the tested fungus were also reported. It is showed that at initial glucose concentration of 20gL-1, Richard-modified logistic model (R2 =0.9839) for microbial growth, modified Gompertz model (R2 =0.9610) for product formation and first order non-growth associate (R2 =0.9736) for glucose consumption were fittd well with the experimental data. Sequential optimization strategy based on one-factor-at-a-time (OFAT) method and statistical experimental design were employed to optimize the process parameters for the enhancement of Ag nanoparticles synthesis. Based on the OFAT method, three significant factors influencing the size of Ag nanoparticles such as AgNO3 concentration, incubation temperature and agitation speed 2014-02 Thesis http://eprints.usm.my/46389/ http://eprints.usm.my/46389/1/Chan%20Yen%20San24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Chan, Yen San Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
description	Controlling sizes of nanomaterials are attracting a great deal of attention because of their unit properties for achieving specific processes especially in biological and medical applications. In this context, Ag which possesses good antimicrobial effects is of interest. A simple and effective approach for the synthesis of Ag nanoparticles by reduction mechanism of proteins synthesized by several species of white rot fungi was studied. Pycnoporus sanguineus was found to produce smallest Ag nanoparticles with degree of monodispersity. A Box-Behnken design was employed to optimize a fermentation medium for the production of protein by the selected fungi. The four variables involved in the study were glucose, malt extract, yeast extract and pH. The optimized medium contained 8.39 gL-1, 18.34 gL-1, 14.71 gL-1 of yeast extract, glucose and malt extract respectively with initial pH 6.66; and produced the maximum protein at 0.858 gL-1. Kinetic growth of the fungi, glucose consumption and product formation of the tested fungus were also reported. It is showed that at initial glucose concentration of 20gL-1, Richard-modified logistic model (R2 =0.9839) for microbial growth, modified Gompertz model (R2 =0.9610) for product formation and first order non-growth associate (R2 =0.9736) for glucose consumption were fittd well with the experimental data. Sequential optimization strategy based on one-factor-at-a-time (OFAT) method and statistical experimental design were employed to optimize the process parameters for the enhancement of Ag nanoparticles synthesis. Based on the OFAT method, three significant factors influencing the size of Ag nanoparticles such as AgNO3 concentration, incubation temperature and agitation speed
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Chan, Yen San
author_facet	Chan, Yen San
author_sort	Chan, Yen San
title	Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
title_short	Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
title_full	Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
title_fullStr	Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
title_full_unstemmed	Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
title_sort	protein enrichment of pycnoporus sanguineus for silver nanoparticles synthesis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2014
url	http://eprints.usm.my/46389/1/Chan%20Yen%20San24.pdf
_version_	1747821660983001088

Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis

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