Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector

Capillary electrophoresis (CE) method employing native β-cyclodextrin (β-CD) and modified hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral selectors was studied and compared for simultaneous enantioseparation of two imidazole antifungal agents. The two imidazole antifungals agents which were ketocon...

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Main Author:	Azhari, Nurul Raihana
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/46400/1/NURUL%20RAIHANA%20BINTI%20AZHARI_HJ.pdf
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spelling	my-usm-ep.464002020-02-28T08:45:11Z Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector 2019-07 Azhari, Nurul Raihana R Medicine (General) Capillary electrophoresis (CE) method employing native β-cyclodextrin (β-CD) and modified hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral selectors was studied and compared for simultaneous enantioseparation of two imidazole antifungal agents. The two imidazole antifungals agents which were ketoconazole and miconazole were successfully resolved with good resolution (Rs: 1.6 and 3.7, respectively) within 16 min using 1.5 mM of HP-β-CD in the background electrolyte (BGE) containing 35 mM of tris-phosphate buffer (pH 2.5) at the 25 ºC and 25 kV. The samples were injected hydrodynamically at 50 mbar for 5 sec and detection was carried out at 200 nm. The method of each enantiomer was linear over the the concentration ranges of 0.25 – 50 mg L-1 with high coefficient of determination (R2 > 0.999) and the limit of determination (LOD) and limit of quantification (LOQ) were 0.075 and 0.250 mg L-1, respectively. The proposed method was also applied to selected antifungal cream samples and good recoveries were found, ranging from 82.0 – 105.7%, (RSD < 7%, n = 3). In addition, in order to understand in depth the possible chiral separation mechanism of both imidazole compounds with β-CD and HP-β-CD, the formation of inclusion complexes were analyzed using 1H NMR spectroscopy and UV-Vis spectrophotometry techniques and lastly, proposed structure of inclusion complexes were drawn and presented. 2019-07 Thesis http://eprints.usm.my/46400/ http://eprints.usm.my/46400/1/NURUL%20RAIHANA%20BINTI%20AZHARI_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Azhari, Nurul Raihana Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
description	Capillary electrophoresis (CE) method employing native β-cyclodextrin (β-CD) and modified hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral selectors was studied and compared for simultaneous enantioseparation of two imidazole antifungal agents. The two imidazole antifungals agents which were ketoconazole and miconazole were successfully resolved with good resolution (Rs: 1.6 and 3.7, respectively) within 16 min using 1.5 mM of HP-β-CD in the background electrolyte (BGE) containing 35 mM of tris-phosphate buffer (pH 2.5) at the 25 ºC and 25 kV. The samples were injected hydrodynamically at 50 mbar for 5 sec and detection was carried out at 200 nm. The method of each enantiomer was linear over the the concentration ranges of 0.25 – 50 mg L-1 with high coefficient of determination (R2 > 0.999) and the limit of determination (LOD) and limit of quantification (LOQ) were 0.075 and 0.250 mg L-1, respectively. The proposed method was also applied to selected antifungal cream samples and good recoveries were found, ranging from 82.0 – 105.7%, (RSD < 7%, n = 3). In addition, in order to understand in depth the possible chiral separation mechanism of both imidazole compounds with β-CD and HP-β-CD, the formation of inclusion complexes were analyzed using 1H NMR spectroscopy and UV-Vis spectrophotometry techniques and lastly, proposed structure of inclusion complexes were drawn and presented.
format	Thesis
qualification_level	Master's degree
author	Azhari, Nurul Raihana
author_facet	Azhari, Nurul Raihana
author_sort	Azhari, Nurul Raihana
title	Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
title_short	Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
title_full	Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
title_fullStr	Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
title_full_unstemmed	Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector
title_sort	enantioseparation of ketoconazole and miconazole by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as a chiral selector
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2019
url	http://eprints.usm.my/46400/1/NURUL%20RAIHANA%20BINTI%20AZHARI_HJ.pdf
_version_	1747821662930206720

Enantioseparation Of Ketoconazole And Miconazole By Capillary Electrophoresis Using Hydroxypropyl-Β-Cyclodextrin As A Chiral Selector

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