The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7)
Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of va...
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my-usm-ep.464232020-03-04T03:13:25Z The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) 2019-09 Fisall, Umiey Fahietah Mohd R Medicine (General) Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. An antioxidant contents in DF-CME-MOL for DPPH and ABTS assay were 10.52±0.009 mg/g TEAC and 4.32±0.021 mg/g TEAC, respectively. While, total phenolic and flavonoid content were 34.96±0.006 mg/g GAE and 15.72±0.050 mg/g QUE, respectively. After that, compound analysis by GC-MS detected some potential compounds in DF-CME-MOL named; Phenol, 4-(methoxymethyl)-, 2-Methoxy-4-vinylphenol, Phenol, 2-propyl, Phenol, 2-methoxy-4-(1-propenyl)-, (Z)-, 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol, Hexadecanoid acid, methyl ester, Phenol, 2-[(4-hydroxyphenyl) methyl], Phenol, 4, 4‟-methylenebis-, Phenol, 2-ethyl-, Benzeneethanol, 4-hydroxy-, Benzaldehyde, 4-hydroxy-, Benzeneacetonitrile, 4-hydroxy- and Benzaldehyde, 3,4-dimethoxy-. Then, apoptosis analysis was carried out by using Annexin V-FITC through flow cytometry FACS and further confirm by Western blot in detected the signalling of apoptosis pathway using selected proteins such as p53, Bax, Cytochrome c and Caspase 8. The DF-CME-MOL greatly induced early apoptosis through extrinsic pathway in MCF-7 cells. Thus, MOL has a potential to be developed into anticancer agent on breast cancer. 2019-09 Thesis http://eprints.usm.my/46423/ http://eprints.usm.my/46423/1/AHMAD%20ZAERI%20BIN%20LATAHIR_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R Medicine (General) |
| spellingShingle |
R Medicine (General) Fisall, Umiey Fahietah Mohd The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| description |
Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. An antioxidant contents in DF-CME-MOL for DPPH and ABTS assay were 10.52±0.009 mg/g TEAC and 4.32±0.021 mg/g TEAC, respectively. While, total phenolic and flavonoid content were 34.96±0.006 mg/g GAE and 15.72±0.050 mg/g QUE, respectively. After that, compound analysis by GC-MS detected some potential compounds in DF-CME-MOL named; Phenol, 4-(methoxymethyl)-, 2-Methoxy-4-vinylphenol, Phenol, 2-propyl, Phenol, 2-methoxy-4-(1-propenyl)-, (Z)-, 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol, Hexadecanoid acid, methyl ester, Phenol, 2-[(4-hydroxyphenyl) methyl], Phenol, 4, 4‟-methylenebis-, Phenol, 2-ethyl-, Benzeneethanol, 4-hydroxy-, Benzaldehyde, 4-hydroxy-, Benzeneacetonitrile, 4-hydroxy- and Benzaldehyde, 3,4-dimethoxy-. Then, apoptosis analysis was carried out by using Annexin V-FITC through flow cytometry FACS and further confirm by Western blot in detected the signalling of apoptosis pathway using selected proteins such as p53, Bax, Cytochrome c and Caspase 8. The DF-CME-MOL greatly induced early apoptosis through extrinsic pathway in MCF-7 cells. Thus, MOL has a potential to be developed into anticancer agent on breast cancer. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Fisall, Umiey Fahietah Mohd |
| author_facet |
Fisall, Umiey Fahietah Mohd |
| author_sort |
Fisall, Umiey Fahietah Mohd |
| title |
The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| title_short |
The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| title_full |
The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| title_fullStr |
The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| title_full_unstemmed |
The Effects Of Moringa Oleifera Leave Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (MCF-7) |
| title_sort |
effects of moringa oleifera leave extract on cytotoxicity and apoptosis pathway in breast cancer cell (mcf-7) |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Perubatan & Pergigian Termaju |
| publishDate |
2019 |
| url |
http://eprints.usm.my/46423/1/AHMAD%20ZAERI%20BIN%20LATAHIR_HJ.pdf |
| _version_ |
1747821667595321344 |