Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)

The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and...

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Main Author:	Foong, Shin Yee
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf
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id	my-usm-ep.46425
record_format	uketd_dc
spelling	my-usm-ep.464252020-03-04T06:28:54Z Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit) 2014-02 Foong, Shin Yee TP1-1185 Chemical technology The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and 99.5% of the isolates, respectively. The volatile profiles of both fruits were quite similar, both of which being dominated by alcohols (H. polyrhizus, 36.5%; H. undatus, 38.6%) and carboxylic acids (H. polyrhizus, 35.1%; H. undatus, 22.3%), with 1-hexanol and squalene as the two major components. The volatile constituents of H. polyrhizus showed stronger antibacterial activity than those of H. undatus when tested against five bacteria (Bacillus subtilis subsp. spizizenii, Escherichia coli, Klebsiella pneumonia, Pseudomonas stutzeri and Staphylococcus aureus) using the micro-dilution antibacterial assay. This is the first report for the volatile constituents and antibacterial acitivities of the both Hylocereus species. The phytochemical investigation on H. polyrhizus had led to the isolation and identification of six compounds. Myo-inositol (C1) was isolated from the methanolic fruit extract while octatriacont-1-ene (C2), lupeol (C3), stigmasterol (C4), a mixture of stigmasterol and β-sitosterol (C5) and quercetin (C6) were isolated from the chloroform extract of the stems. The structures of these compounds were elucidated by spectroscopic techniques such as IR, DP-MS, 1D-NMR, 2D-NMR and UV-Vis. 2014-02 Thesis http://eprints.usm.my/46425/ http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Foong, Shin Yee Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
description	The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and 99.5% of the isolates, respectively. The volatile profiles of both fruits were quite similar, both of which being dominated by alcohols (H. polyrhizus, 36.5%; H. undatus, 38.6%) and carboxylic acids (H. polyrhizus, 35.1%; H. undatus, 22.3%), with 1-hexanol and squalene as the two major components. The volatile constituents of H. polyrhizus showed stronger antibacterial activity than those of H. undatus when tested against five bacteria (Bacillus subtilis subsp. spizizenii, Escherichia coli, Klebsiella pneumonia, Pseudomonas stutzeri and Staphylococcus aureus) using the micro-dilution antibacterial assay. This is the first report for the volatile constituents and antibacterial acitivities of the both Hylocereus species. The phytochemical investigation on H. polyrhizus had led to the isolation and identification of six compounds. Myo-inositol (C1) was isolated from the methanolic fruit extract while octatriacont-1-ene (C2), lupeol (C3), stigmasterol (C4), a mixture of stigmasterol and β-sitosterol (C5) and quercetin (C6) were isolated from the chloroform extract of the stems. The structures of these compounds were elucidated by spectroscopic techniques such as IR, DP-MS, 1D-NMR, 2D-NMR and UV-Vis.
format	Thesis
qualification_level	Master's degree
author	Foong, Shin Yee
author_facet	Foong, Shin Yee
author_sort	Foong, Shin Yee
title	Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_short	Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_full	Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_fullStr	Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_full_unstemmed	Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_sort	phytochemical studies on hylocereus polyrhizus (fruit and stem) and hylocereus undatus (fruit)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2014
url	http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf
_version_	1747821668080812032

Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)

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