Identification And Characterization Of Fusarium Species From Highlands In Malaysia

Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in th...

全面介紹

Saved in:
書目詳細資料
主要作者: Manshor, Nurhazrati
格式: Thesis
語言:English
出版: 2014
主題:
在線閱讀:http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf
標簽: 添加標簽
沒有標簽, 成為第一個標記此記錄!
id my-usm-ep.46451
record_format uketd_dc
spelling my-usm-ep.464512020-03-06T03:09:11Z Identification And Characterization Of Fusarium Species From Highlands In Malaysia 2014-02 Manshor, Nurhazrati QH1-278.5 Natural history (General) Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in the temperate and subtropical regions were also reported to be in the highlands of tropical areas, including Malaysia. This research was to identify Fusarium species in context of occurrence, diversity, and toxigenicity. Phase sampling was done randomly from variable hosts and substrate in three locations, Cameron Highlands, Fraser’s Hill and Kinabalu Park. The samples were plated on a semi-selective medium (pentachloronitrobenzene agar) and incubated in a standard incubation conditions (27+1°C). One-hundred twenty three (123) Fusarium isolates were single-spored and identified based on the morphological characteristics. Four species were identified i.e. F. graminearum (86.2%), followed by F. venenatum (12.2%), F. avenaceum (0.8%) and F. sporotrichioides (0.8%). Molecular characterization and mycotoxin profiles were used to support the morphological data. The molecular characterization were carried out by using PCR-RFLP of the rDNA intergenic spacer (IGS) region and DNA sequences using Translation α-Elongation Factor (TEF) gene. BLAST search for sequence similarity by using GenBank database which demonstrated 88% - 99% whereas for Fusarium Database was between 89% - 99%. 2014-02 Thesis http://eprints.usm.my/46451/ http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Manshor, Nurhazrati
Identification And Characterization Of Fusarium Species From Highlands In Malaysia
description Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in the temperate and subtropical regions were also reported to be in the highlands of tropical areas, including Malaysia. This research was to identify Fusarium species in context of occurrence, diversity, and toxigenicity. Phase sampling was done randomly from variable hosts and substrate in three locations, Cameron Highlands, Fraser’s Hill and Kinabalu Park. The samples were plated on a semi-selective medium (pentachloronitrobenzene agar) and incubated in a standard incubation conditions (27+1°C). One-hundred twenty three (123) Fusarium isolates were single-spored and identified based on the morphological characteristics. Four species were identified i.e. F. graminearum (86.2%), followed by F. venenatum (12.2%), F. avenaceum (0.8%) and F. sporotrichioides (0.8%). Molecular characterization and mycotoxin profiles were used to support the morphological data. The molecular characterization were carried out by using PCR-RFLP of the rDNA intergenic spacer (IGS) region and DNA sequences using Translation α-Elongation Factor (TEF) gene. BLAST search for sequence similarity by using GenBank database which demonstrated 88% - 99% whereas for Fusarium Database was between 89% - 99%.
format Thesis
qualification_level Master's degree
author Manshor, Nurhazrati
author_facet Manshor, Nurhazrati
author_sort Manshor, Nurhazrati
title Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_short Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_full Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_fullStr Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_full_unstemmed Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_sort identification and characterization of fusarium species from highlands in malaysia
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2014
url http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf
_version_ 1747821673694887936