Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule

Eighteen fruit samples from Cucurbitaceae family that are commonly found in Malaysia, were selected in this study. All extracts were evaluated for their antioxidant activities and total phenolic contents. The primary antioxidant activities were evaluated using 1, 1 diphenyl-2-picrylhydrazyl (DPPH) f...

Full description

Saved in:

Bibliographic Details
Main Author:	Supriatno, Supriatno
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/46458/1/Supriatno24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.46458
record_format	uketd_dc
spelling	my-usm-ep.464582020-03-06T08:54:23Z Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule 2013-11 Supriatno, Supriatno TP1-1185 Chemical technology Eighteen fruit samples from Cucurbitaceae family that are commonly found in Malaysia, were selected in this study. All extracts were evaluated for their antioxidant activities and total phenolic contents. The primary antioxidant activities were evaluated using 1, 1 diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and reducing power assays, and the secondary antioxidant activity was determined using metal chelating assay. For fruits that are edible in raw form, they were freshly extracted, whereas for the cooking vegetables, they were being boiled prior to extraction. Different percentages of ethanol in distilled water (0, 20, 40, 60, 80 and 100%) were used as the extraction solvents and 80% (v/v) ethanol extract of Lagenaria siceraria showed the highest DPPH, reducing power and metal chelating activities with the EC50 values of 8.85 ± 0.09 mg/mL, 28.33 ± 1.67 mg/mL, and 6.51 ± 0.17 mg/mL, respectively. Furthermore, four solvents of ascending polarities (hexane, chloroform, ethyl acetate and methanol) were being used to sequentially extract the dried fruit samples. The methanol and ethyl acetate extracts of L. siceraria respectively showed the highest DPPH (EC50 = 370.01 ± 12.51 and 469.00 ± 2.89 μg/mL) and metal chelating activities (EC50 = 249.78 ± 5.03 and 203.44 ± 4.01 μg/mL), while the ethyl acetate extract of Sechium edule showed the highest reducing power (EC50 = 409.35 ± 9.25 μg/mL). 2013-11 Thesis http://eprints.usm.my/46458/ http://eprints.usm.my/46458/1/Supriatno24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Supriatno, Supriatno Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
description	Eighteen fruit samples from Cucurbitaceae family that are commonly found in Malaysia, were selected in this study. All extracts were evaluated for their antioxidant activities and total phenolic contents. The primary antioxidant activities were evaluated using 1, 1 diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and reducing power assays, and the secondary antioxidant activity was determined using metal chelating assay. For fruits that are edible in raw form, they were freshly extracted, whereas for the cooking vegetables, they were being boiled prior to extraction. Different percentages of ethanol in distilled water (0, 20, 40, 60, 80 and 100%) were used as the extraction solvents and 80% (v/v) ethanol extract of Lagenaria siceraria showed the highest DPPH, reducing power and metal chelating activities with the EC50 values of 8.85 ± 0.09 mg/mL, 28.33 ± 1.67 mg/mL, and 6.51 ± 0.17 mg/mL, respectively. Furthermore, four solvents of ascending polarities (hexane, chloroform, ethyl acetate and methanol) were being used to sequentially extract the dried fruit samples. The methanol and ethyl acetate extracts of L. siceraria respectively showed the highest DPPH (EC50 = 370.01 ± 12.51 and 469.00 ± 2.89 μg/mL) and metal chelating activities (EC50 = 249.78 ± 5.03 and 203.44 ± 4.01 μg/mL), while the ethyl acetate extract of Sechium edule showed the highest reducing power (EC50 = 409.35 ± 9.25 μg/mL).
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Supriatno, Supriatno
author_facet	Supriatno, Supriatno
author_sort	Supriatno, Supriatno
title	Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
title_short	Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
title_full	Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
title_fullStr	Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
title_full_unstemmed	Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule
title_sort	antioxidant and alpha glucosidase inhibitory activities of extracts from cucurbit fruits and identification of active and major constituents from phenolic-rich extracts of lagenaria siceraria and sechium edule
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2013
url	http://eprints.usm.my/46458/1/Supriatno24.pdf
_version_	1747821675410358272

Antioxidant And Alpha Glucosidase Inhibitory Activities Of Extracts From Cucurbit Fruits And Identification Of Active And Major Constituents From Phenolic-Rich Extracts Of Lagenaria Siceraria And Sechium Edule

Similar Items