The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the a...
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2014
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my-usm-ep.464732020-03-09T03:44:31Z The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells 2014-01 Utar, Zulkhurnain RK1-715 Dentistry Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems). 2014-01 Thesis http://eprints.usm.my/46473/ http://eprints.usm.my/46473/1/Zulkhurnain%20Bin%20Utar24.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju |
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Universiti Sains Malaysia |
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USM Institutional Repository |
| language |
English |
| topic |
RK1-715 Dentistry |
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RK1-715 Dentistry Utar, Zulkhurnain The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells |
| description |
Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems). |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Utar, Zulkhurnain |
| author_facet |
Utar, Zulkhurnain |
| author_sort |
Utar, Zulkhurnain |
| title |
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_short |
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_full |
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_fullStr |
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_full_unstemmed |
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_sort |
effects of mitragynine from
mitragyna speciosa on the mrna
expression of cox-1 and cox-2 in
lipopolysaccharide-stimulated
raw264.7 macrophage cells |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Perubatan & Pergigian Termaju |
| publishDate |
2014 |
| url |
http://eprints.usm.my/46473/1/Zulkhurnain%20Bin%20Utar24.pdf |
| _version_ |
1747821679091908608 |