The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells

The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells

Propolis is one of the bee products which contained multiple chemical compounds, namely polyphenols, waxes, steroids and terpenoids. Its contents may be influenced by the plant sources, geographical area as well as the bee species. A wide array of natural products have been used as anti-inflammatory...

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Main Author: Asem, NorNaimah
Format: Thesis
Language:English
Published: 2019
Subjects:
R Medicine
Online Access:http://eprints.usm.my/46490/1/NORNAIMAH%20ASEM_HJ.pdf
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spelling my-usm-ep.464902020-03-12T02:19:16Z The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells 2019-08 Asem, NorNaimah R Medicine Propolis is one of the bee products which contained multiple chemical compounds, namely polyphenols, waxes, steroids and terpenoids. Its contents may be influenced by the plant sources, geographical area as well as the bee species. A wide array of natural products have been used as anti-inflammatory and healing agents, with propolis being a remarkable option. Therefore, the objective of this study is to examine the phytochemical properties of the selected propolis extracts and its effects on the expression of adhesion molecules, as well as the molecular mechanisms involved, particularly the investigations on the nuclear factor-kappa B (NF-ĸB) signalling pathways in cultured endothelial cell. In the present study, the phytochemical screening of ethanolic extract of propolis (EEP) from local stingless bee species namely Tetrigona apicalis, Geniotrigona thoracica and Heterotrigona itama were investigated using high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analysis. The total phenolic and flavonoid contents, and antioxidant activities which include 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay were explored. 2019-08 Thesis http://eprints.usm.my/46490/ http://eprints.usm.my/46490/1/NORNAIMAH%20ASEM_HJ.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R Medicine
spellingShingle R Medicine
Asem, NorNaimah
The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
description Propolis is one of the bee products which contained multiple chemical compounds, namely polyphenols, waxes, steroids and terpenoids. Its contents may be influenced by the plant sources, geographical area as well as the bee species. A wide array of natural products have been used as anti-inflammatory and healing agents, with propolis being a remarkable option. Therefore, the objective of this study is to examine the phytochemical properties of the selected propolis extracts and its effects on the expression of adhesion molecules, as well as the molecular mechanisms involved, particularly the investigations on the nuclear factor-kappa B (NF-ĸB) signalling pathways in cultured endothelial cell. In the present study, the phytochemical screening of ethanolic extract of propolis (EEP) from local stingless bee species namely Tetrigona apicalis, Geniotrigona thoracica and Heterotrigona itama were investigated using high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analysis. The total phenolic and flavonoid contents, and antioxidant activities which include 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay were explored.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Asem, NorNaimah
author_facet Asem, NorNaimah
author_sort Asem, NorNaimah
title The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
title_short The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
title_full The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
title_fullStr The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
title_full_unstemmed The Effect Of Propolis Extract From Trigona Spp. On Vascular Inflammatory Mediators And Its Molecular Mechanism In TNF-Alpha-Stimulated Endothelial Cells
title_sort effect of propolis extract from trigona spp. on vascular inflammatory mediators and its molecular mechanism in tnf-alpha-stimulated endothelial cells
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2019
url http://eprints.usm.my/46490/1/NORNAIMAH%20ASEM_HJ.pdf
_version_ 1747821682752487424

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