Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines

Breast cancer is one of the common malignancies among women worldwide. There is a constant demand for new therapies to treat this complex disease. Notably, scientific research is drawing its attention towards medicinal plants as a potential source for anticancer agents. Christia vespertilionis is...

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Main Author:	Zakaria, Muhammad Asyaari
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RG Gynecology and obstetrics
Online Access:	http://eprints.usm.my/46581/1/Dr.%20Muhammad%20Asyaari%20Zakaria-24%20pages.pdf
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id	my-usm-ep.46581
record_format	uketd_dc
spelling	my-usm-ep.465812020-06-18T04:21:51Z Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines 2018-12 Zakaria, Muhammad Asyaari RG Gynecology and obstetrics Breast cancer is one of the common malignancies among women worldwide. There is a constant demand for new therapies to treat this complex disease. Notably, scientific research is drawing its attention towards medicinal plants as a potential source for anticancer agents. Christia vespertilionis is a local herb claimed to have anticancer properties. It is widely marketed as a food supplement in Malaysia thus raise an awareness regarding their efficacy and safety. Moreover, current research about the mechanisms of its anticancer potential is also limited. Herein, this study aimed to evaluate the efficacy of C. vespertilionis extracts on breast cancer cell lines (MDAMB- 231 and MCF-7) and to investigate the mode of cell death that underlies its anticancer effects. Besides, the phytochemical in CVME and heavy metals of the plant were also investigated. The cytotoxic activities of CVME, CVAE and tamoxifen was evaluated by MTT assay while the mode of cell death was evaluated by Annexin VFITC and PI staining. The phytochemical and heavy metal analysis were investigated by GC-MS and ICP-MS, respectively. Results from MTT assay showed that CVME significantly inhibits proliferation of MDA-MB-231 cells line (p<0.05) and the effect was selectively cytotoxic towards cancerous cells only. Furthermore, this study showed that CVME induces apoptosis as indicated by a significant increase of apoptotic cell percentage (p<0.05) when compared with untreated cells. CVME was also found to contain numbers of pharmacologically bioactive compounds that possess anticancer activities such as phytol and 10-undecenoic acid. Hazardous heavy metal analysis revealed that this plant contains a high concentration of chromium which may cause toxic side effects. Overall, this study demonstrates the potential applications of C. vespertilionis as an anticancer drug for breast cancer treatment. Pusat Pengajian Sains Perubatan 2018-12 Thesis http://eprints.usm.my/46581/ http://eprints.usm.my/46581/1/Dr.%20Muhammad%20Asyaari%20Zakaria-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan,
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Zakaria, Muhammad Asyaari Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
description	Breast cancer is one of the common malignancies among women worldwide. There is a constant demand for new therapies to treat this complex disease. Notably, scientific research is drawing its attention towards medicinal plants as a potential source for anticancer agents. Christia vespertilionis is a local herb claimed to have anticancer properties. It is widely marketed as a food supplement in Malaysia thus raise an awareness regarding their efficacy and safety. Moreover, current research about the mechanisms of its anticancer potential is also limited. Herein, this study aimed to evaluate the efficacy of C. vespertilionis extracts on breast cancer cell lines (MDAMB- 231 and MCF-7) and to investigate the mode of cell death that underlies its anticancer effects. Besides, the phytochemical in CVME and heavy metals of the plant were also investigated. The cytotoxic activities of CVME, CVAE and tamoxifen was evaluated by MTT assay while the mode of cell death was evaluated by Annexin VFITC and PI staining. The phytochemical and heavy metal analysis were investigated by GC-MS and ICP-MS, respectively. Results from MTT assay showed that CVME significantly inhibits proliferation of MDA-MB-231 cells line (p<0.05) and the effect was selectively cytotoxic towards cancerous cells only. Furthermore, this study showed that CVME induces apoptosis as indicated by a significant increase of apoptotic cell percentage (p<0.05) when compared with untreated cells. CVME was also found to contain numbers of pharmacologically bioactive compounds that possess anticancer activities such as phytol and 10-undecenoic acid. Hazardous heavy metal analysis revealed that this plant contains a high concentration of chromium which may cause toxic side effects. Overall, this study demonstrates the potential applications of C. vespertilionis as an anticancer drug for breast cancer treatment.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Muhammad Asyaari
author_facet	Zakaria, Muhammad Asyaari
author_sort	Zakaria, Muhammad Asyaari
title	Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
title_short	Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
title_full	Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
title_fullStr	Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
title_full_unstemmed	Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
title_sort	safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan,
publishDate	2018
url	http://eprints.usm.my/46581/1/Dr.%20Muhammad%20Asyaari%20Zakaria-24%20pages.pdf
_version_	1747821689848201216

Safety and efficacy evaluation of christia vespertilionis extracts on breast cancer cell lines

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