Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells

Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyse the chemical profile of the propolis extract and to quantify the major chemical compounds of t...

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Main Author:	Rosli, Nur’liyana
Format:	Thesis
Language:	English
Published:	2019
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/46603/1/Complete%20THESIS%20%28MASTER%2924.pdf
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id	my-usm-ep.46603
record_format	uketd_dc
spelling	my-usm-ep.466032020-06-24T04:03:05Z Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells 2019-02 Rosli, Nur’liyana RK1-715 Dentistry Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyse the chemical profile of the propolis extract and to quantify the major chemical compounds of the propolis extract using total phenolic and total flavonoid content methods, to determine the antioxidant activities of the propolis extract using DPPH and ABTS colourimetric assays, and to assess the wound healing properties of propolis extract using scratch assay. The ethanolic extract of propolis was subjected to HPLC analysis to analyse its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH and ABTS radical scavenging assay and FRAP assay. TPC and TFC were performed to determine the correlation with its antioxidant activities which show the strong correlation, r2 > 0.9. Scratch assay was later tested for wound healing properties in the gingival fibroblast cell lines based on dose dependent and time dependent manner. The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 for DPPH and ABTS assay were 1.90 mg/ml and 0.57 mg/ml, respectively. Correlation values of TPC and TFC against DPPH, ABTS and FRAP indicated that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid content. 2019-02 Thesis http://eprints.usm.my/46603/ http://eprints.usm.my/46603/1/Complete%20THESIS%20%28MASTER%2924.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Rosli, Nur’liyana Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
description	Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyse the chemical profile of the propolis extract and to quantify the major chemical compounds of the propolis extract using total phenolic and total flavonoid content methods, to determine the antioxidant activities of the propolis extract using DPPH and ABTS colourimetric assays, and to assess the wound healing properties of propolis extract using scratch assay. The ethanolic extract of propolis was subjected to HPLC analysis to analyse its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH and ABTS radical scavenging assay and FRAP assay. TPC and TFC were performed to determine the correlation with its antioxidant activities which show the strong correlation, r2 > 0.9. Scratch assay was later tested for wound healing properties in the gingival fibroblast cell lines based on dose dependent and time dependent manner. The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 for DPPH and ABTS assay were 1.90 mg/ml and 0.57 mg/ml, respectively. Correlation values of TPC and TFC against DPPH, ABTS and FRAP indicated that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid content.
format	Thesis
qualification_level	Master's degree
author	Rosli, Nur’liyana
author_facet	Rosli, Nur’liyana
author_sort	Rosli, Nur’liyana
title	Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
title_short	Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
title_full	Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
title_fullStr	Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
title_full_unstemmed	Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells
title_sort	effects of trigona apicalis propolis ethanolic extract on antioxidant activities and wound healing using human gingival fibroblast cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2019
url	http://eprints.usm.my/46603/1/Complete%20THESIS%20%28MASTER%2924.pdf
_version_	1747821694516461568

Effects Of Trigona Apicalis Propolis Ethanolic Extract On Antioxidant Activities And Wound Healing Using Human Gingival Fibroblast Cells

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