Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin

High demands for Dendrobium cut flowers as well as the secondary metabolite products lead to the over-exploitation of the orchid from its natural habitat. The use of in vitro hybrid orchid is one of a prominent strategy to meet the demand. Dendrobium Sabin Blue (a hybrid species between Dendrobium B...

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Main Author:	Malik, Aimie Nadzirah Abd
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46619/1/Nadzirah%2024.pdf
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id	my-usm-ep.46619
record_format	uketd_dc
spelling	my-usm-ep.466192020-06-25T07:48:06Z Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin 2019-08 Malik, Aimie Nadzirah Abd QH1-278.5 Natural history (General) High demands for Dendrobium cut flowers as well as the secondary metabolite products lead to the over-exploitation of the orchid from its natural habitat. The use of in vitro hybrid orchid is one of a prominent strategy to meet the demand. Dendrobium Sabin Blue (a hybrid species between Dendrobium Blue Angel and Dendrobium Sanan Blue) orchid was used in this study to produce anthocyanin. The aim of this study is to improve in vitro proliferation of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue and enhancing anthocyanin content by using elicitors. For PLBs proliferation, four different types of plant growth regulators (1-Naphthaleneacetic acid, 6-Benzylaminopurine, kinetin and picloram) with different concentrations were tested. The results revealed that 10 μM of NAA produced the highest growth index of PLBs with 10.947 ± 0.825 as compared to the control with only 1.67 ± 0.111. In addition, histological analysis showed a dense meristematic cell and presence of starch grains which indicates the actively growing of cell. In addition, a delicate technique was utilized to further promote a rapid and mass propagation of PLBs through in vitro culture of thin cell layer (TCL). After four weeks of culture, results showed that transverse TCL (tTCL) produced the highest number of PLBs with a total of 59 PLBs were produced from a single thin layer cut as compared to longitudinal TCL (lTCL). The tTCL also produced significantly higher number of shoots with 15 shoots as the highest record. 2019-08 Thesis http://eprints.usm.my/46619/ http://eprints.usm.my/46619/1/Nadzirah%2024.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Malik, Aimie Nadzirah Abd Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
description	High demands for Dendrobium cut flowers as well as the secondary metabolite products lead to the over-exploitation of the orchid from its natural habitat. The use of in vitro hybrid orchid is one of a prominent strategy to meet the demand. Dendrobium Sabin Blue (a hybrid species between Dendrobium Blue Angel and Dendrobium Sanan Blue) orchid was used in this study to produce anthocyanin. The aim of this study is to improve in vitro proliferation of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue and enhancing anthocyanin content by using elicitors. For PLBs proliferation, four different types of plant growth regulators (1-Naphthaleneacetic acid, 6-Benzylaminopurine, kinetin and picloram) with different concentrations were tested. The results revealed that 10 μM of NAA produced the highest growth index of PLBs with 10.947 ± 0.825 as compared to the control with only 1.67 ± 0.111. In addition, histological analysis showed a dense meristematic cell and presence of starch grains which indicates the actively growing of cell. In addition, a delicate technique was utilized to further promote a rapid and mass propagation of PLBs through in vitro culture of thin cell layer (TCL). After four weeks of culture, results showed that transverse TCL (tTCL) produced the highest number of PLBs with a total of 59 PLBs were produced from a single thin layer cut as compared to longitudinal TCL (lTCL). The tTCL also produced significantly higher number of shoots with 15 shoots as the highest record.
format	Thesis
qualification_level	Master's degree
author	Malik, Aimie Nadzirah Abd
author_facet	Malik, Aimie Nadzirah Abd
author_sort	Malik, Aimie Nadzirah Abd
title	Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
title_short	Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
title_full	Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
title_fullStr	Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
title_full_unstemmed	Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin
title_sort	proliferation and elicitation of protocorm-like bodies of dendrobium sabin blue for in vitro production of anthocyanin
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/46619/1/Nadzirah%2024.pdf
_version_	1747821698175991808

Proliferation And Elicitation Of Protocorm-Like Bodies Of Dendrobium Sabin Blue For In Vitro Production Of Anthocyanin

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