Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr

Chemical fingerprints are commonly used to perform the authentication and identification of Chinese herbal medicines (CHM). During the last few years, the fingerprint method has been developed for quality control of Chinese herbal medicines. Fingerprinting is a method that evaluates the characterist...

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Main Author:	Ng, Chiew Hoong
Format:	Thesis
Language:	English
Published:	2019
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/46620/1/Ng%20Chiew%20Hoong24.pdf
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id	my-usm-ep.46620
record_format	uketd_dc
spelling	my-usm-ep.466202020-06-25T07:51:22Z Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr 2019-06 Ng, Chiew Hoong RS1-441 Pharmacy and materia medica Chemical fingerprints are commonly used to perform the authentication and identification of Chinese herbal medicines (CHM). During the last few years, the fingerprint method has been developed for quality control of Chinese herbal medicines. Fingerprinting is a method that evaluates the characteristic pattern of the ingredients using one or more identification techniques. Therefore, in this study, the spectroscopic and chromatographic fingerprints of Gelsemium elegans were studied by using four analytical instruments, namely the HPTLC, LC-MS, FT-IR tri-step identification, and NMR. The purpose of this study was to compare different analytical methods on Gelsemium elegans from Fu Jian and Guang Xi province as the object of study to distinguish between the stem, leaf and root as they contained different amounts of indole alkaloid that contributes to its toxicity. The differentiation between the different parts was difficult as they came from the same species and shared similar properties and active compounds. However, the three different parts of Gelsemium elegans was successfully distinguished by the four instruments. Besides that, two abundant active compound present in Gelsemium elegans, gelsemine and koumine was qualitatively studied in HPTLC and LC-PDA-QDa. Furthermore, LC-MS/MS conditions were also developed for quantitative determination of gelsemine and koumine in Gelsemium elegans. The content of Koumine was also used to cross check the quantity in LC-MS/MS by HPTLC. Both methods showed good precision, recovery, stability and repeatability. 2019-06 Thesis http://eprints.usm.my/46620/ http://eprints.usm.my/46620/1/Ng%20Chiew%20Hoong24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Ng, Chiew Hoong Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
description	Chemical fingerprints are commonly used to perform the authentication and identification of Chinese herbal medicines (CHM). During the last few years, the fingerprint method has been developed for quality control of Chinese herbal medicines. Fingerprinting is a method that evaluates the characteristic pattern of the ingredients using one or more identification techniques. Therefore, in this study, the spectroscopic and chromatographic fingerprints of Gelsemium elegans were studied by using four analytical instruments, namely the HPTLC, LC-MS, FT-IR tri-step identification, and NMR. The purpose of this study was to compare different analytical methods on Gelsemium elegans from Fu Jian and Guang Xi province as the object of study to distinguish between the stem, leaf and root as they contained different amounts of indole alkaloid that contributes to its toxicity. The differentiation between the different parts was difficult as they came from the same species and shared similar properties and active compounds. However, the three different parts of Gelsemium elegans was successfully distinguished by the four instruments. Besides that, two abundant active compound present in Gelsemium elegans, gelsemine and koumine was qualitatively studied in HPTLC and LC-PDA-QDa. Furthermore, LC-MS/MS conditions were also developed for quantitative determination of gelsemine and koumine in Gelsemium elegans. The content of Koumine was also used to cross check the quantity in LC-MS/MS by HPTLC. Both methods showed good precision, recovery, stability and repeatability.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ng, Chiew Hoong
author_facet	Ng, Chiew Hoong
author_sort	Ng, Chiew Hoong
title	Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
title_short	Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
title_full	Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
title_fullStr	Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
title_full_unstemmed	Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr
title_sort	spectroscopic and chromatographic approach for the chemical fingerprinting of gelsemium elegans via hptlc, lc-ms, ft-ir and nmr
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2019
url	http://eprints.usm.my/46620/1/Ng%20Chiew%20Hoong24.pdf
_version_	1747821698419261440

Spectroscopic And Chromatographic Approach For The Chemical Fingerprinting Of Gelsemium Elegans Via Hptlc, Lc-Ms, Ft-Ir And Nmr

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