Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion

Cerebral hypoperfusion is a condition where there is reduced blood flow to the brain. This condition is observed in patients with cardiovascular diseases, hypertension and among the aging population. Cerebral hypoperfusion leads to more severe neurological diseases including vascular dementia, Alzhe...

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Main Author:	Sekaran, Hema
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/46629/1/thesis%20final%20print%20%28one%20file%29_hema_1704201924.pdf
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id	my-usm-ep.46629
record_format	uketd_dc
spelling	my-usm-ep.466292020-06-26T08:27:52Z Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion 2019-04 Sekaran, Hema R5-920 Medicine (General) Cerebral hypoperfusion is a condition where there is reduced blood flow to the brain. This condition is observed in patients with cardiovascular diseases, hypertension and among the aging population. Cerebral hypoperfusion leads to more severe neurological diseases including vascular dementia, Alzheimer’s disease and multiple sclerosis. Cerebral hypoperfusion induces damages to the blood-brain barrier (BBB) and this detailed study was carried out to understand the BBB behavior during cerebral hypoperfusion. In this study, two-vessel occlusion (2VO) model was used to mimic cerebral hypoperfusion in rats. Using exogenous tracers of different molecular weight i.e. Evans blue dye (69 kDa) as a marker for macromolecule leakage, and sodium fluorescein (376 Da) as a marker for small molecule leakage, BBB leakages to Evans blue dye was observed during the early phase of cerebral hypoperfusion, 1-day post-surgery in the brain regions frontal cortex, posterior cortex and thalamus-midbrain. Using transmission electron microscopy, vesicles and formation of fenestration in endothelial cells, and swelling of astrocyte end-feet were observed, indicating cellular damages leading to BBB leakages during cerebral hypoperfusion. 2019-04 Thesis http://eprints.usm.my/46629/ http://eprints.usm.my/46629/1/thesis%20final%20print%20%28one%20file%29_hema_1704201924.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Penyelidikan Dadah dan Ubat-ubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Sekaran, Hema Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
description	Cerebral hypoperfusion is a condition where there is reduced blood flow to the brain. This condition is observed in patients with cardiovascular diseases, hypertension and among the aging population. Cerebral hypoperfusion leads to more severe neurological diseases including vascular dementia, Alzheimer’s disease and multiple sclerosis. Cerebral hypoperfusion induces damages to the blood-brain barrier (BBB) and this detailed study was carried out to understand the BBB behavior during cerebral hypoperfusion. In this study, two-vessel occlusion (2VO) model was used to mimic cerebral hypoperfusion in rats. Using exogenous tracers of different molecular weight i.e. Evans blue dye (69 kDa) as a marker for macromolecule leakage, and sodium fluorescein (376 Da) as a marker for small molecule leakage, BBB leakages to Evans blue dye was observed during the early phase of cerebral hypoperfusion, 1-day post-surgery in the brain regions frontal cortex, posterior cortex and thalamus-midbrain. Using transmission electron microscopy, vesicles and formation of fenestration in endothelial cells, and swelling of astrocyte end-feet were observed, indicating cellular damages leading to BBB leakages during cerebral hypoperfusion.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Sekaran, Hema
author_facet	Sekaran, Hema
author_sort	Sekaran, Hema
title	Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
title_short	Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
title_full	Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
title_fullStr	Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
title_full_unstemmed	Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion
title_sort	changes of the blood-brain barrier permeability, ultrastructure, and protein and gene expressions in a rat model of cerebral hypoperfusion
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Dadah dan Ubat-ubatan
publishDate	2019
url	http://eprints.usm.my/46629/1/thesis%20final%20print%20%28one%20file%29_hema_1704201924.pdf
_version_	1747821700360175616

Changes Of The Blood-Brain Barrier Permeability, Ultrastructure, And Protein And Gene Expressions In A Rat Model Of Cerebral Hypoperfusion

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