Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A

Post-translational modification (PTM) of protein is known to play a major role in protein function. One of the most important PTM is ubiquitination, in which the ubiquitin molecule(s) are added to a target protein by ubiquitin activating enzymes (E1s), ubiquitin conjugating enzymes (E2s) and ubiquit...

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Main Author:	Iskandar, Athirah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/46638/1/ATHIRAH%20BINTI%20ISKANDAR_HJ.pdf
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id	my-usm-ep.46638
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spelling	my-usm-ep.466382020-06-29T02:20:47Z Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A 2019 Iskandar, Athirah R5-920 Medicine (General) Post-translational modification (PTM) of protein is known to play a major role in protein function. One of the most important PTM is ubiquitination, in which the ubiquitin molecule(s) are added to a target protein by ubiquitin activating enzymes (E1s), ubiquitin conjugating enzymes (E2s) and ubiquitin ligases (E3s). This process is reversible by the action of specialized enzymes known as deubiquitinases (DUBs). OTUB1 is a DUBs that was reported to has critical roles in cancer initiation and progression. To investigate the role of OTUB1 in breast cancer, this preliminary study was conducted to compare the expression of OTUB1 in human breast adenocarcinoma cell line, MCF7 and human epithelial cell line, MCF10A. Expression of OTUB1 in MCF7 and MCF10A was analysed by quantitative real-time PCR and relative quantification (RQ) value was obtained using ΔΔCt method. Mann-Whitney test was used to evaluate the differences in OTUB1 expression levels between MCF7 and MCF10A. To support our qPCR study, computational analysis was also performed on selected microarray datasets from Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). qPCR analysis shows that OTUB1 expression was significantly higher in MCF7 compared to MCF10A (p = 0.037). Computational analysis on selected GEO microarray datasets shows that almost all probes corresponding to OTUB1 confirmed that OTUB1 expression is significantly higher in MCF7. Therefore, further studies should be done to uncover the role and function of OTUB1 in cancer-associated pathways. 2019 Thesis http://eprints.usm.my/46638/ http://eprints.usm.my/46638/1/ATHIRAH%20BINTI%20ISKANDAR_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Iskandar, Athirah Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
description	Post-translational modification (PTM) of protein is known to play a major role in protein function. One of the most important PTM is ubiquitination, in which the ubiquitin molecule(s) are added to a target protein by ubiquitin activating enzymes (E1s), ubiquitin conjugating enzymes (E2s) and ubiquitin ligases (E3s). This process is reversible by the action of specialized enzymes known as deubiquitinases (DUBs). OTUB1 is a DUBs that was reported to has critical roles in cancer initiation and progression. To investigate the role of OTUB1 in breast cancer, this preliminary study was conducted to compare the expression of OTUB1 in human breast adenocarcinoma cell line, MCF7 and human epithelial cell line, MCF10A. Expression of OTUB1 in MCF7 and MCF10A was analysed by quantitative real-time PCR and relative quantification (RQ) value was obtained using ΔΔCt method. Mann-Whitney test was used to evaluate the differences in OTUB1 expression levels between MCF7 and MCF10A. To support our qPCR study, computational analysis was also performed on selected microarray datasets from Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). qPCR analysis shows that OTUB1 expression was significantly higher in MCF7 compared to MCF10A (p = 0.037). Computational analysis on selected GEO microarray datasets shows that almost all probes corresponding to OTUB1 confirmed that OTUB1 expression is significantly higher in MCF7. Therefore, further studies should be done to uncover the role and function of OTUB1 in cancer-associated pathways.
format	Thesis
qualification_level	Master's degree
author	Iskandar, Athirah
author_facet	Iskandar, Athirah
author_sort	Iskandar, Athirah
title	Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
title_short	Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
title_full	Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
title_fullStr	Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
title_full_unstemmed	Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A
title_sort	analysis of otub1 expression in human breast adenocarcinoma cell line mcf7 and human breast epithelial cell line mcf10a
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2019
url	http://eprints.usm.my/46638/1/ATHIRAH%20BINTI%20ISKANDAR_HJ.pdf
_version_	1747821702326255616

Analysis Of OTUB1 Expression In Human Breast Adenocarcinoma Cell Line MCF7 And Human Breast Epithelial Cell Line MCF10A

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