Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines

Cancer is one of the leading causes of mortality and morbidity all over the world. Various types of cancer-causing genetic aberrations are well characterized such as mutations, gene amplification, translocation, structural deletion and chromosomal mis-segregation. With the use of modern genomic tech...

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Main Author:	Noor, Nor Shila Mohamed
Format:	Thesis
Language:	English
Published:	2019
Subjects:	RC254-282 Neoplasms Tumors Oncology (including Cancer)
Online Access:	http://eprints.usm.my/46642/1/NOR%20SHILA%20MOHAMED%20NOOR_HJ.pdf
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id	my-usm-ep.46642
record_format	uketd_dc
spelling	my-usm-ep.466422020-06-29T07:40:01Z Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines 2019 Noor, Nor Shila Mohamed RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Cancer is one of the leading causes of mortality and morbidity all over the world. Various types of cancer-causing genetic aberrations are well characterized such as mutations, gene amplification, translocation, structural deletion and chromosomal mis-segregation. With the use of modern genomic technologies, we are now beginning to understand the enormous complexity of cancer. The growing understanding of cancer cell biology and tumor progression is gradually leading to better methods for treating the disease. The emergence of genome editing tools offers an exciting solution to addressing this issue by disrupting expression of cancer genes. In this study, the current genome editing tool, CRISPR/Cas9 system, is used to demonstrate its potential in disrupting the expression of OTUB1 gene in breast, bone and liver cancer cells. OTUB1 gene is ubiquitously expressed in human tissues and play an important role in many physiological and pathological processes of human. OTUB1 is also strongly correlated with cancer progression. Prior to transfection to knockdown OTUB1, optimization of transfection procedure and transfection efficacy in cell lines need to be done to get optimum results. Analysis using fluorescence microscopy and flow cytometry showed that Lipofectamine 3000 is a preferable method in transfection. These encouraging results will enable us to conduct further investigations on exploring the role of OTUB1 gene in signalling pathways of different cancer cells. These results showed that the recombinant DNA successfully constructed to knockdown OTUB1 using CRISPR/Cas9 system can be a promising tool for cancer treatment strategies in the future. 2019 Thesis http://eprints.usm.my/46642/ http://eprints.usm.my/46642/1/NOR%20SHILA%20MOHAMED%20NOOR_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC254-282 Neoplasms Tumors Oncology (including Cancer)
spellingShingle	RC254-282 Neoplasms Tumors Oncology (including Cancer) Noor, Nor Shila Mohamed Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
description	Cancer is one of the leading causes of mortality and morbidity all over the world. Various types of cancer-causing genetic aberrations are well characterized such as mutations, gene amplification, translocation, structural deletion and chromosomal mis-segregation. With the use of modern genomic technologies, we are now beginning to understand the enormous complexity of cancer. The growing understanding of cancer cell biology and tumor progression is gradually leading to better methods for treating the disease. The emergence of genome editing tools offers an exciting solution to addressing this issue by disrupting expression of cancer genes. In this study, the current genome editing tool, CRISPR/Cas9 system, is used to demonstrate its potential in disrupting the expression of OTUB1 gene in breast, bone and liver cancer cells. OTUB1 gene is ubiquitously expressed in human tissues and play an important role in many physiological and pathological processes of human. OTUB1 is also strongly correlated with cancer progression. Prior to transfection to knockdown OTUB1, optimization of transfection procedure and transfection efficacy in cell lines need to be done to get optimum results. Analysis using fluorescence microscopy and flow cytometry showed that Lipofectamine 3000 is a preferable method in transfection. These encouraging results will enable us to conduct further investigations on exploring the role of OTUB1 gene in signalling pathways of different cancer cells. These results showed that the recombinant DNA successfully constructed to knockdown OTUB1 using CRISPR/Cas9 system can be a promising tool for cancer treatment strategies in the future.
format	Thesis
qualification_level	Master's degree
author	Noor, Nor Shila Mohamed
author_facet	Noor, Nor Shila Mohamed
author_sort	Noor, Nor Shila Mohamed
title	Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
title_short	Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
title_full	Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
title_fullStr	Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
title_full_unstemmed	Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines
title_sort	construction of recombinant otbu1 knockdown in breast, bone and liver cancer cell lines
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2019
url	http://eprints.usm.my/46642/1/NOR%20SHILA%20MOHAMED%20NOOR_HJ.pdf
_version_	1747821703051870208

Construction Of Recombinant OTBU1 Knockdown In Breast, Bone And Liver Cancer Cell Lines

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