The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)

The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)

Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of va...

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Main Author: Fisall, Umiey Fahietah Mohd
Format: Thesis
Language:English
Published: 2019
Subjects:
RK1-715 Dentistry
Online Access:http://eprints.usm.my/46682/1/UMIEY%20FAHIETAH24.pdf
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spelling my-usm-ep.466822020-07-03T07:59:06Z The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) 2019-08 Fisall, Umiey Fahietah Mohd RK1-715 Dentistry Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. 2019-08 Thesis http://eprints.usm.my/46682/ http://eprints.usm.my/46682/1/UMIEY%20FAHIETAH24.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RK1-715 Dentistry
spellingShingle RK1-715 Dentistry
Fisall, Umiey Fahietah Mohd
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
description Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis.
format Thesis
qualification_level Master's degree
author Fisall, Umiey Fahietah Mohd
author_facet Fisall, Umiey Fahietah Mohd
author_sort Fisall, Umiey Fahietah Mohd
title The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
title_short The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
title_full The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
title_fullStr The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
title_full_unstemmed The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
title_sort effect of moringa oleifera leaf extract on cytotoxicity and apoptosis pathway in breast cancer cell (mcf-7)
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2019
url http://eprints.usm.my/46682/1/UMIEY%20FAHIETAH24.pdf
_version_ 1747821711842082816

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