The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of va...
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my-usm-ep.466822020-07-03T07:59:06Z The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) 2019-08 Fisall, Umiey Fahietah Mohd RK1-715 Dentistry Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. 2019-08 Thesis http://eprints.usm.my/46682/ http://eprints.usm.my/46682/1/UMIEY%20FAHIETAH24.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
RK1-715 Dentistry |
| spellingShingle |
RK1-715 Dentistry Fisall, Umiey Fahietah Mohd The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| description |
Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Fisall, Umiey Fahietah Mohd |
| author_facet |
Fisall, Umiey Fahietah Mohd |
| author_sort |
Fisall, Umiey Fahietah Mohd |
| title |
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| title_short |
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| title_full |
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| title_fullStr |
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| title_full_unstemmed |
The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) |
| title_sort |
effect of moringa oleifera leaf extract on cytotoxicity and apoptosis pathway in breast cancer cell (mcf-7) |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Perubatan & Pergigian Termaju |
| publishDate |
2019 |
| url |
http://eprints.usm.my/46682/1/UMIEY%20FAHIETAH24.pdf |
| _version_ |
1747821711842082816 |