In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen

In recent years, injectable hydrogel has shown great potential for biomedical applications owing to its distinct properties of in situ gelation. In this research, ultrasonication was introduced to pretreat tendon collagen used in the project to reduce the size of fibrils and prevent fibril aggregati...

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Main Author:	Lau, Sin Mun
Format:	Thesis
Language:	English
Published:	2019
Subjects:	T Technology TP155-156 Chemical engineering
Online Access:	http://eprints.usm.my/46697/1/In%20Situ%20Enzymatic%20Synthesis%20Of%20Collagen-Chitosan%20Hydrogel%20Using%20Ultrasonic%20Retreated%20Collagen.pdf
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spelling	my-usm-ep.466972021-11-17T03:42:12Z In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen 2019-02-01 Lau, Sin Mun T Technology TP155-156 Chemical engineering In recent years, injectable hydrogel has shown great potential for biomedical applications owing to its distinct properties of in situ gelation. In this research, ultrasonication was introduced to pretreat tendon collagen used in the project to reduce the size of fibrils and prevent fibril aggregation. The pretreated collagen (Col) was combined with chitosan (Chit) to improve the physical properties of hydrogels. Composite hydrogel was formed by oxidative coupling of the phenolic hydroxyl (Ph) groups in polymer chains using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The resulting Col-Chit-Ph solutions were able to form rapid hydrogel via peroxidase-catalyzed crosslinking reaction. The concentration of HRP and H2O2 affected the gelation time of Col-Chit-Ph hydrogels. Besides, the Col to Chit (Col:Chit) ratio also significantly influenced the physical, in vitro and mechanical characteristics of composite hydrogels. The hydrogel with a higher composition of chitosan has faster gelation, higher mechanical strength, and lower degradability, but resulted in lower porosity of the hydrogel matrix. The results obtained in this study reveal the importance of the chitosan ratio to the Col-Chit-Ph injectable hydrogel composite. The most significant results were obtained in the conjugate hydrogel with Col:Chit ratio of 3:2, which has the highest cell growth rate compared to others after 5 days of incubations. Hence, this hydrogel has the potential for use in the biomedical fields as it is mechanically stable and supports cellular functions. 2019-02 Thesis http://eprints.usm.my/46697/ http://eprints.usm.my/46697/1/In%20Situ%20Enzymatic%20Synthesis%20Of%20Collagen-Chitosan%20Hydrogel%20Using%20Ultrasonic%20Retreated%20Collagen.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T Technology TP155-156 Chemical engineering
spellingShingle	T Technology TP155-156 Chemical engineering Lau, Sin Mun In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
description	In recent years, injectable hydrogel has shown great potential for biomedical applications owing to its distinct properties of in situ gelation. In this research, ultrasonication was introduced to pretreat tendon collagen used in the project to reduce the size of fibrils and prevent fibril aggregation. The pretreated collagen (Col) was combined with chitosan (Chit) to improve the physical properties of hydrogels. Composite hydrogel was formed by oxidative coupling of the phenolic hydroxyl (Ph) groups in polymer chains using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The resulting Col-Chit-Ph solutions were able to form rapid hydrogel via peroxidase-catalyzed crosslinking reaction. The concentration of HRP and H2O2 affected the gelation time of Col-Chit-Ph hydrogels. Besides, the Col to Chit (Col:Chit) ratio also significantly influenced the physical, in vitro and mechanical characteristics of composite hydrogels. The hydrogel with a higher composition of chitosan has faster gelation, higher mechanical strength, and lower degradability, but resulted in lower porosity of the hydrogel matrix. The results obtained in this study reveal the importance of the chitosan ratio to the Col-Chit-Ph injectable hydrogel composite. The most significant results were obtained in the conjugate hydrogel with Col:Chit ratio of 3:2, which has the highest cell growth rate compared to others after 5 days of incubations. Hence, this hydrogel has the potential for use in the biomedical fields as it is mechanically stable and supports cellular functions.
format	Thesis
qualification_level	Master's degree
author	Lau, Sin Mun
author_facet	Lau, Sin Mun
author_sort	Lau, Sin Mun
title	In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
title_short	In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
title_full	In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
title_fullStr	In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
title_full_unstemmed	In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen
title_sort	in situ enzymatic synthesis of collagen-chitosan hydrogel using ultrasonic retreated collagen
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2019
url	http://eprints.usm.my/46697/1/In%20Situ%20Enzymatic%20Synthesis%20Of%20Collagen-Chitosan%20Hydrogel%20Using%20Ultrasonic%20Retreated%20Collagen.pdf
_version_	1747821714304139264

In Situ Enzymatic Synthesis Of Collagen-Chitosan Hydrogel Using Ultrasonic Retreated Collagen

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