The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action

Haemoglobin metabolism during the intraerythrocytic stage of the malaria parasite, Plasmodium falciparum is a preferable target for artemisinin, which is widely used for treatment and control of malaria. It has previously been reported that the parasite ingests haemoglobin via mouth-like structur...

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Main Author: Zamri, Noor Hafizah Mohd
Format: Thesis
Language:English
Published: 2018
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Online Access:http://eprints.usm.my/46755/1/Dr.%20Noor%20Hafizah%20Mohd%20Zamri-24%20pages.pdf
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spelling my-usm-ep.467552020-07-19T01:40:58Z The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action 2018-04 Zamri, Noor Hafizah Mohd RA643-645 Disease (Communicable and noninfectious) and public health Haemoglobin metabolism during the intraerythrocytic stage of the malaria parasite, Plasmodium falciparum is a preferable target for artemisinin, which is widely used for treatment and control of malaria. It has previously been reported that the parasite ingests haemoglobin via mouth-like structures named cytostomes. Haemoglobin-containing vesicles that bud off from cytostomes are transported to the digestive vacuole (DV) where the haemoglobin is degraded. However, the details of endocytic process as well as how artemisinin affects this process are still debatable. Hence, in this study we re-examined the endocytic process of the parasite and observed the effect of artemisinin on this process by using an endocytic marker, tetramethylrhodamine-dextran (TMR-dextran) and a pH indicator, Lysosensor Blue (LB) and SNARF-1-dextran. Resealed erythrocytes containing TMR-dextran were prepared at an optimised ratio of 1:3 of erythrocytes to haemolysis buffer volume. The resealed erythrocytes permitted retention of 33.56 ± 7.84% of the original haemoglobin contents and showed comparable parasite‟s invasion efficiency to normal erythrocytes. An early endocytic event of the parasites was initiated at mid ring stage with appearance of typical small endocytic compartments and a large spherical structure termed as a “big gulp”. An acidic DV concentrated with TMRdextran and labelled with LB, appeared at the later stages of trophozoite and schizont. Artemisinin treatments on this process showed no modification on thehaemoglobin intake by the parasite and no alterations of the LB label and SNARF-1- dextran of the DV indicating no major pH changes of the vacuole. In conclusion, haemoglobin is ingested by the parasite at early intraerythrocytic stage and accumulated in the acidic DV where the vacuole showed no significant pH alterations upon the artemisinin treatments. 2018-04 Thesis http://eprints.usm.my/46755/ http://eprints.usm.my/46755/1/Dr.%20Noor%20Hafizah%20Mohd%20Zamri-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RA643-645 Disease (Communicable and noninfectious) and public health
spellingShingle RA643-645 Disease (Communicable and noninfectious) and public health
Zamri, Noor Hafizah Mohd
The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
description Haemoglobin metabolism during the intraerythrocytic stage of the malaria parasite, Plasmodium falciparum is a preferable target for artemisinin, which is widely used for treatment and control of malaria. It has previously been reported that the parasite ingests haemoglobin via mouth-like structures named cytostomes. Haemoglobin-containing vesicles that bud off from cytostomes are transported to the digestive vacuole (DV) where the haemoglobin is degraded. However, the details of endocytic process as well as how artemisinin affects this process are still debatable. Hence, in this study we re-examined the endocytic process of the parasite and observed the effect of artemisinin on this process by using an endocytic marker, tetramethylrhodamine-dextran (TMR-dextran) and a pH indicator, Lysosensor Blue (LB) and SNARF-1-dextran. Resealed erythrocytes containing TMR-dextran were prepared at an optimised ratio of 1:3 of erythrocytes to haemolysis buffer volume. The resealed erythrocytes permitted retention of 33.56 ± 7.84% of the original haemoglobin contents and showed comparable parasite‟s invasion efficiency to normal erythrocytes. An early endocytic event of the parasites was initiated at mid ring stage with appearance of typical small endocytic compartments and a large spherical structure termed as a “big gulp”. An acidic DV concentrated with TMRdextran and labelled with LB, appeared at the later stages of trophozoite and schizont. Artemisinin treatments on this process showed no modification on thehaemoglobin intake by the parasite and no alterations of the LB label and SNARF-1- dextran of the DV indicating no major pH changes of the vacuole. In conclusion, haemoglobin is ingested by the parasite at early intraerythrocytic stage and accumulated in the acidic DV where the vacuole showed no significant pH alterations upon the artemisinin treatments.
format Thesis
qualification_level Master's degree
author Zamri, Noor Hafizah Mohd
author_facet Zamri, Noor Hafizah Mohd
author_sort Zamri, Noor Hafizah Mohd
title The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
title_short The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
title_full The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
title_fullStr The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
title_full_unstemmed The feeding process of Plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
title_sort feeding process of plasmodium falciparum in resealed erythrocytes containing endocytic markers and its role in artemisinin action
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Perubatan
publishDate 2018
url http://eprints.usm.my/46755/1/Dr.%20Noor%20Hafizah%20Mohd%20Zamri-24%20pages.pdf
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