Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)

Breast cancer is one of the major contributions in non-communicable diseases worldwide. To date, much evidence has shown that resistance to apoptosis by cancer cells and inefficient efferocytosis by phagocytic cells in cancer patients foster the development of other diseases in cancer patients, s...

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Main Author:	Muhamad, Nor ‘Atiqah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RG Gynecology and obstetrics
Online Access:	http://eprints.usm.my/46768/1/Dr.%20Nor%20Atiqah%20Muhamad-24%20pages.pdf
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id	my-usm-ep.46768
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spelling	my-usm-ep.467682020-07-21T03:48:18Z Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA) 2018-01 Muhamad, Nor ‘Atiqah RG Gynecology and obstetrics Breast cancer is one of the major contributions in non-communicable diseases worldwide. To date, much evidence has shown that resistance to apoptosis by cancer cells and inefficient efferocytosis by phagocytic cells in cancer patients foster the development of other diseases in cancer patients, such as autoimmune diseases. Previous studies have shown that Centella asiatica (C. asiatica) extract induced apoptosis in cancer cell line but there was a lack of study regarding efferocytosis against cancer cells. C. asiatica has the potential to be an alternative treatment to induce apoptosis and efferocytosis by phagocytic cells in cancer patients. The anti-proliferative activity of methanol extract of C. asiatica (MECA) against breast cancer cell line (MCF-7) was obtained by performing methylene blue assay (MBA). In this method, DMSO served as a negative control while tamoxifen served as a positive control. After that, the mode of cell death in MECA-treated cells was determined by Hoechst 33342 staining and was further investigated by flow cytometry analysis. The findings on the MBA suggested that MECA was cytotoxic on MCF-7 and not toxic on normal cells (NIH3T3). Furthermore, MCF-7 treated with MECA undergone increased apoptosis indicated by brighter fluorescence, alteration of nuclear morphology and DNA fragmentation observed in Hoechst staining. To study efferocytosis activity by macrophages (J774A.1), MCF-7 cells were cultivated in J774A.1 cells with multiplicity of infection (MOI) of 1:2. After treatment with MECA, flow cytometry data showed a significant increase of late apoptotic cells due to apoptosis of J774A.1 after engulfing MCF-7 cells. In conclusion, MECA was able to improve the activities of apoptosis and efferocytosis in MCF-7 cells, thus suggesting a promising alternative treatment for breast cancer. 2018-01 Thesis http://eprints.usm.my/46768/ http://eprints.usm.my/46768/1/Dr.%20Nor%20Atiqah%20Muhamad-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Muhamad, Nor ‘Atiqah Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
description	Breast cancer is one of the major contributions in non-communicable diseases worldwide. To date, much evidence has shown that resistance to apoptosis by cancer cells and inefficient efferocytosis by phagocytic cells in cancer patients foster the development of other diseases in cancer patients, such as autoimmune diseases. Previous studies have shown that Centella asiatica (C. asiatica) extract induced apoptosis in cancer cell line but there was a lack of study regarding efferocytosis against cancer cells. C. asiatica has the potential to be an alternative treatment to induce apoptosis and efferocytosis by phagocytic cells in cancer patients. The anti-proliferative activity of methanol extract of C. asiatica (MECA) against breast cancer cell line (MCF-7) was obtained by performing methylene blue assay (MBA). In this method, DMSO served as a negative control while tamoxifen served as a positive control. After that, the mode of cell death in MECA-treated cells was determined by Hoechst 33342 staining and was further investigated by flow cytometry analysis. The findings on the MBA suggested that MECA was cytotoxic on MCF-7 and not toxic on normal cells (NIH3T3). Furthermore, MCF-7 treated with MECA undergone increased apoptosis indicated by brighter fluorescence, alteration of nuclear morphology and DNA fragmentation observed in Hoechst staining. To study efferocytosis activity by macrophages (J774A.1), MCF-7 cells were cultivated in J774A.1 cells with multiplicity of infection (MOI) of 1:2. After treatment with MECA, flow cytometry data showed a significant increase of late apoptotic cells due to apoptosis of J774A.1 after engulfing MCF-7 cells. In conclusion, MECA was able to improve the activities of apoptosis and efferocytosis in MCF-7 cells, thus suggesting a promising alternative treatment for breast cancer.
format	Thesis
qualification_level	Master's degree
author	Muhamad, Nor ‘Atiqah
author_facet	Muhamad, Nor ‘Atiqah
author_sort	Muhamad, Nor ‘Atiqah
title	Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
title_short	Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
title_full	Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
title_fullStr	Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
title_full_unstemmed	Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)
title_sort	clearance of apoptotic cells by macrophage in mcf-7 cell line treated with methanol extract of centella asiatica (meca)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/46768/1/Dr.%20Nor%20Atiqah%20Muhamad-24%20pages.pdf
_version_	1747821725068820480

Clearance of apoptotic cells by macrophage in MCF-7 cell line treated with methanol extract of centella asiatica (MECA)

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