Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis

The Orang Asli population represent only about 0.5% of total population of Malaysia. There are 18 sub-groups of Orang Asli that belong to the Semang, Senoi and Proto-Malay. These sub-groups have diverse cultures, traditions, beliefs and languages depending on their ethnicity and location. In this...

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Main Author:	Abdullah, Nur Azeelah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/47042/1/Dr.%20Nur%20Azeelah%20Abdullah-24%20pages.pdf
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spelling	my-usm-ep.470422020-09-01T03:02:55Z Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis 2018-03 Abdullah, Nur Azeelah R Medicine The Orang Asli population represent only about 0.5% of total population of Malaysia. There are 18 sub-groups of Orang Asli that belong to the Semang, Senoi and Proto-Malay. These sub-groups have diverse cultures, traditions, beliefs and languages depending on their ethnicity and location. In this study, a total of 164 blood samples from six Orang Asli sub-groups in Peninsular Malaysia; Semai, Che Wong, Orang Kanaq, Lanoh, Bateq and Kensiu were PCR-type for 15 autosomal STR loci using Identifiler Direct system and 12 X-chromosomal STR loci using Argus X-12 QS system. The allele frequencies and several forensic parameters for STR as well as the genetic structure in the Orang Asli sub-groups were determined. The agreement with Hardy-weinberg Equilibrium (HWE) was confirmed for all loci in Semai and Lanoh sub-groups for autosomal STR results and Che Wong for X-STR results. The combined power of exclusion (CPE) were higher than 0.999. The combined power of discrimination (CPD) also supports the theory that no two individuals would have the same genotype except for identical twins. FGA is the most variable and informative marker for forensic testing of the 15 autosomal STR loci while DXS10146 is the most informative marker for X-STR system of the Orang Asli in Peninsular Malaysia. The Orang Asli sub-groups were plotted closed to each other in the Principal Component Analysis (PCA), suggesting some degree of common ancestry between the subgroups.These findings indicate the uniqueness of the Orang Asli sub-groups and the evidence of their ancient genetic affinities as they represent the first human migration wave out of Africa. The STR and X-STR profile dataset are important for forensic applications and for establishment of the Orang Asli population data in Peninsular Malaysia, which are currently not available for this population. 2018-03 Thesis http://eprints.usm.my/47042/ http://eprints.usm.my/47042/1/Dr.%20Nur%20Azeelah%20Abdullah-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Abdullah, Nur Azeelah Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
description	The Orang Asli population represent only about 0.5% of total population of Malaysia. There are 18 sub-groups of Orang Asli that belong to the Semang, Senoi and Proto-Malay. These sub-groups have diverse cultures, traditions, beliefs and languages depending on their ethnicity and location. In this study, a total of 164 blood samples from six Orang Asli sub-groups in Peninsular Malaysia; Semai, Che Wong, Orang Kanaq, Lanoh, Bateq and Kensiu were PCR-type for 15 autosomal STR loci using Identifiler Direct system and 12 X-chromosomal STR loci using Argus X-12 QS system. The allele frequencies and several forensic parameters for STR as well as the genetic structure in the Orang Asli sub-groups were determined. The agreement with Hardy-weinberg Equilibrium (HWE) was confirmed for all loci in Semai and Lanoh sub-groups for autosomal STR results and Che Wong for X-STR results. The combined power of exclusion (CPE) were higher than 0.999. The combined power of discrimination (CPD) also supports the theory that no two individuals would have the same genotype except for identical twins. FGA is the most variable and informative marker for forensic testing of the 15 autosomal STR loci while DXS10146 is the most informative marker for X-STR system of the Orang Asli in Peninsular Malaysia. The Orang Asli sub-groups were plotted closed to each other in the Principal Component Analysis (PCA), suggesting some degree of common ancestry between the subgroups.These findings indicate the uniqueness of the Orang Asli sub-groups and the evidence of their ancient genetic affinities as they represent the first human migration wave out of Africa. The STR and X-STR profile dataset are important for forensic applications and for establishment of the Orang Asli population data in Peninsular Malaysia, which are currently not available for this population.
format	Thesis
qualification_level	Master's degree
author	Abdullah, Nur Azeelah
author_facet	Abdullah, Nur Azeelah
author_sort	Abdullah, Nur Azeelah
title	Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
title_short	Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
title_full	Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
title_fullStr	Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
title_full_unstemmed	Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis
title_sort	genetic variations of six orang asli sub-groups in peninsular malaysia using autosomal and x-chromosome short tandem repeats (strs) analysis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/47042/1/Dr.%20Nur%20Azeelah%20Abdullah-24%20pages.pdf
_version_	1747821751085039616

Genetic variations of six orang asli sub-groups in Peninsular Malaysia using autosomal and x-chromosome short tandem repeats (STRs) analysis

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