Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10

Oil palm frond (OPF) is one of the most abundant waste generated from an oil palm plantation. OPF can be utilized as a feedstock for bioethanol and can simultaneously address its disposal issue in the plantation. In this project, OPF was divided into two types of feedstock; biomass and juice by m...

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Main Author:	Halim, Farah Amani Abdul
Format:	Thesis
Language:	English
Published:	2018
Subjects:	T175-178 Industrial research Research and development
Online Access:	http://eprints.usm.my/47497/1/FARAH%20AMANI%20ABDUL%20HALIM%20-%20PRETREATMENT%20OF%20OIL%20PALM%20FROND%20BY%20Phanerochaete%20crysosporium%20CK01%20AND%20ENZYMATIC%20HYDROLYSIS%20FOR%20ETHANOL%20PRODUCTION%20BY%20Saccharomyces%20cerevisiae%20HC10.pdf
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spelling	my-usm-ep.474972020-10-12T06:47:22Z Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10 2018-08 Halim, Farah Amani Abdul T175-178 Industrial research. Research and development Oil palm frond (OPF) is one of the most abundant waste generated from an oil palm plantation. OPF can be utilized as a feedstock for bioethanol and can simultaneously address its disposal issue in the plantation. In this project, OPF was divided into two types of feedstock; biomass and juice by mechanical pressing. Firstly, the dried biomass was biologically treated using white-rot fungi; Phanerochaete chrysosporium CK01 for biodelignification. Two parameters were tested; effect of inoculum size and fermentation duration. The best parameters were inoculum size of 1.0x106 spore/mL and 3 weeks of fermentation duration which gave 27.87% of delignification. In the second stage, the treated biomass was subjected to enzymatic hydrolysis by either individual or in combination to produce a sugar hydrolysate. Two parameters tested were enzyme loading (in individual enzymatic hydrolysis) or enzyme ratio (in combined enzymatic hydrolysis; Cellulase A “Amano” 3 and Hemicellulase “Amano” 90) and hydrolysis time. The result shows that the combined (cellulase and hemicellulase) enzymatic hydrolysis produces higher amount of simple sugar (5.15g/L) using a combination of enzyme ratio of 1:4 (cellulase:hemicellulase) with a hydrolysis time of 120 minutes compared to individual enzymatic hydrolysis (3.26g/L). Prior to beginning of the third stage, the amount of simple sugar in the hydrolysate (OPFH) and juice (OPFJ) were compared using high performance liquid chromatography (HPLC) analysis. 2018-08 Thesis http://eprints.usm.my/47497/ http://eprints.usm.my/47497/1/FARAH%20AMANI%20ABDUL%20HALIM%20-%20PRETREATMENT%20OF%20OIL%20PALM%20FROND%20BY%20Phanerochaete%20crysosporium%20CK01%20AND%20ENZYMATIC%20HYDROLYSIS%20FOR%20ETHANOL%20PRODUCTION%20BY%20Saccharomyces%20cerevisiae%20HC10.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T175-178 Industrial research Research and development
spellingShingle	T175-178 Industrial research Research and development Halim, Farah Amani Abdul Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
description	Oil palm frond (OPF) is one of the most abundant waste generated from an oil palm plantation. OPF can be utilized as a feedstock for bioethanol and can simultaneously address its disposal issue in the plantation. In this project, OPF was divided into two types of feedstock; biomass and juice by mechanical pressing. Firstly, the dried biomass was biologically treated using white-rot fungi; Phanerochaete chrysosporium CK01 for biodelignification. Two parameters were tested; effect of inoculum size and fermentation duration. The best parameters were inoculum size of 1.0x106 spore/mL and 3 weeks of fermentation duration which gave 27.87% of delignification. In the second stage, the treated biomass was subjected to enzymatic hydrolysis by either individual or in combination to produce a sugar hydrolysate. Two parameters tested were enzyme loading (in individual enzymatic hydrolysis) or enzyme ratio (in combined enzymatic hydrolysis; Cellulase A “Amano” 3 and Hemicellulase “Amano” 90) and hydrolysis time. The result shows that the combined (cellulase and hemicellulase) enzymatic hydrolysis produces higher amount of simple sugar (5.15g/L) using a combination of enzyme ratio of 1:4 (cellulase:hemicellulase) with a hydrolysis time of 120 minutes compared to individual enzymatic hydrolysis (3.26g/L). Prior to beginning of the third stage, the amount of simple sugar in the hydrolysate (OPFH) and juice (OPFJ) were compared using high performance liquid chromatography (HPLC) analysis.
format	Thesis
qualification_level	Master's degree
author	Halim, Farah Amani Abdul
author_facet	Halim, Farah Amani Abdul
author_sort	Halim, Farah Amani Abdul
title	Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
title_short	Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
title_full	Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
title_fullStr	Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
title_full_unstemmed	Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10
title_sort	pretreatment of oil palm frond by phanerochaete crysosporium ck01 and enzymatic hydrolysis for ethanol production by saccharomyces cerevisiae hc10
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2018
url	http://eprints.usm.my/47497/1/FARAH%20AMANI%20ABDUL%20HALIM%20-%20PRETREATMENT%20OF%20OIL%20PALM%20FROND%20BY%20Phanerochaete%20crysosporium%20CK01%20AND%20ENZYMATIC%20HYDROLYSIS%20FOR%20ETHANOL%20PRODUCTION%20BY%20Saccharomyces%20cerevisiae%20HC10.pdf
_version_	1747821789941071872

Pretreatment Of Oil Palm Frond By Phanerochaete Crysosporium Ck01 And Enzymatic Hydrolysis For Ethanol Production By Saccharomyces Cerevisiae Hc10

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