Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds

Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that t...

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Main Author:	Sofian, Nurul Hidayatul Afifah Saufi
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QK1-989 Botany
Online Access:	http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf
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id	my-usm-ep.47798
record_format	uketd_dc
spelling	my-usm-ep.477982020-10-28T01:04:28Z Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds 2017-12 Sofian, Nurul Hidayatul Afifah Saufi QK1-989 Botany Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that the protein isolates contained a high amount of glutamic acid, glycine, aspartic acid, and leucine with essential to total amino acid ratio of 0.4. The Fourier transform infrared (FTIR) spectra showed that the isolated proteins exhibited a similar functional group of protein, which was extracted from legumes, and the isoelectric point (pI) was found at pH 3-4. The functionalities of both protein isolates were also investigated. The bioactivities were evaluated based on 2,2-diphenyl-1-picrylhydrazyl scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), α-amylase, ACE inhibitory and lipase inhibitory activity assays. 2017-12 Thesis http://eprints.usm.my/47798/ http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Penyelidikan Biokimia Analisis (Analytical Biochemistry Research Centre (ABrC))
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QK1-989 Botany
spellingShingle	QK1-989 Botany Sofian, Nurul Hidayatul Afifah Saufi Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
description	Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that the protein isolates contained a high amount of glutamic acid, glycine, aspartic acid, and leucine with essential to total amino acid ratio of 0.4. The Fourier transform infrared (FTIR) spectra showed that the isolated proteins exhibited a similar functional group of protein, which was extracted from legumes, and the isoelectric point (pI) was found at pH 3-4. The functionalities of both protein isolates were also investigated. The bioactivities were evaluated based on 2,2-diphenyl-1-picrylhydrazyl scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), α-amylase, ACE inhibitory and lipase inhibitory activity assays.
format	Thesis
qualification_level	Master's degree
author	Sofian, Nurul Hidayatul Afifah Saufi
author_facet	Sofian, Nurul Hidayatul Afifah Saufi
author_sort	Sofian, Nurul Hidayatul Afifah Saufi
title	Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
title_short	Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
title_full	Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
title_fullStr	Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
title_full_unstemmed	Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
title_sort	production, characterization and identification of bioactive peptides from annona squamosa l. seeds
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Biokimia Analisis (Analytical Biochemistry Research Centre (ABrC))
publishDate	2017
url	http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf
_version_	1747821831927103488

Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds

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