The study of Interacting Partners of a Transcription Factor, YB-1

The transcription factor, YB-1 was considered to be a transcription factor. YB-1 also involved in many genes regulation, such as genes involved in cell proliferation and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate dependent-transcriptional enhancing, tiss...

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Main Author:	Mansor, Mohamad Hafizi
Format:	Thesis
Language:	English
Published:	2005
Subjects:	RC Internal medicine
Online Access:	http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf
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spelling	my-usm-ep.478432020-11-03T07:49:44Z The study of Interacting Partners of a Transcription Factor, YB-1 2005 Mansor, Mohamad Hafizi RC Internal medicine The transcription factor, YB-1 was considered to be a transcription factor. YB-1 also involved in many genes regulation, such as genes involved in cell proliferation and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate dependent-transcriptional enhancing, tissue cell specific processes, stress response, immune response (major histocompatibility class II genes), and many more. Because of several important of YB-1, my supervisor proposed to study of YB- 1 's interacting partners. Base on interacting partner of YB-1, we hopefully can explore properties of YB-1. In this study, we used culture ofHeLa cells, and lysis the cells using Single-step lysis for extraction of total cellular protein, and nuclear and cytoplasmic proteinextraction methods to get YB-1 protein. Using co-immunoprecipitation analysis and several antibodies, we will detect interacting partners of YB-1. Finally after co-immunoprecipitation analysis, we got YB-1 protein has specific interaction with P-glycoprotein mdr 1 protein which involved in multidrugresistant cancer cells. 2005 Thesis http://eprints.usm.my/47843/ http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Mansor, Mohamad Hafizi The study of Interacting Partners of a Transcription Factor, YB-1
description	The transcription factor, YB-1 was considered to be a transcription factor. YB-1 also involved in many genes regulation, such as genes involved in cell proliferation and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate dependent-transcriptional enhancing, tissue cell specific processes, stress response, immune response (major histocompatibility class II genes), and many more. Because of several important of YB-1, my supervisor proposed to study of YB- 1 's interacting partners. Base on interacting partner of YB-1, we hopefully can explore properties of YB-1. In this study, we used culture ofHeLa cells, and lysis the cells using Single-step lysis for extraction of total cellular protein, and nuclear and cytoplasmic proteinextraction methods to get YB-1 protein. Using co-immunoprecipitation analysis and several antibodies, we will detect interacting partners of YB-1. Finally after co-immunoprecipitation analysis, we got YB-1 protein has specific interaction with P-glycoprotein mdr 1 protein which involved in multidrugresistant cancer cells.
format	Thesis
qualification_level	Master's degree
author	Mansor, Mohamad Hafizi
author_facet	Mansor, Mohamad Hafizi
author_sort	Mansor, Mohamad Hafizi
title	The study of Interacting Partners of a Transcription Factor, YB-1
title_short	The study of Interacting Partners of a Transcription Factor, YB-1
title_full	The study of Interacting Partners of a Transcription Factor, YB-1
title_fullStr	The study of Interacting Partners of a Transcription Factor, YB-1
title_full_unstemmed	The study of Interacting Partners of a Transcription Factor, YB-1
title_sort	study of interacting partners of a transcription factor, yb-1
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2005
url	http://eprints.usm.my/47843/1/The%20Study%20Of%20Interacting%20Partners%20Of%20A%20Transcription%20Factor%2C%20YB-1-24%20pages.pdf
_version_	1747821839009185792

The study of Interacting Partners of a Transcription Factor, YB-1

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