Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities

The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs we...

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Main Author:	Che Mood, Che Nurul Azieyan
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RB37-56.5 Clinical pathology Laboratory technique
Online Access:	http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf
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spelling	my-usm-ep.479222020-11-20T03:23:35Z Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities 2018-07 Che Mood, Che Nurul Azieyan RB37-56.5 Clinical pathology. Laboratory technique The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs were tested on antimicrobial, antioxidant and cytotoxicity activities. The formation of AgNPs was confirmed by UV-Vis spectroscopy at the wavelength of 420 nm (AgNP-L) and 430 nm (AgNP-S). The size were measured at 114 nm (AgNP-L) and 129 nm (AgNP-S) using a zetasizer. From TEM analysis, the size ranged from 10-300 nm and 10-180 nm for AgNP-L and AgNP-S with spherical shape. Zeta potentials recorded at -42.8 mV for AgNP-L and -43.9 mV for AgNP-S. XRD analysis matched the face-centered cubic (fcc) structure of silver. FTIR analysis revealed the presence of few functional groups of phenolic and flavonoid compounds which acts as the reducing agent in AgNPs synthesis. The AgNPs showed good inhibition against bacteria, but not fungus. Inhibition showed that highest against S. aureus with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition against E. coli with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in disc diffusion method. Same trend of result was reported for well diffusion method. In antioxidant assays, AgNP-L showed IC50 at 417.05 μg/mL and AgNP-S at 434.60 μg/mL (DPPH); 304.31 μg/mL (AgNP-L) and 326.83 μg/mL (AgNP-S) for ABTS, respectively. 2018-07 Thesis http://eprints.usm.my/47922/ http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RB37-56.5 Clinical pathology Laboratory technique
spellingShingle	RB37-56.5 Clinical pathology Laboratory technique Che Mood, Che Nurul Azieyan Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
description	The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs were tested on antimicrobial, antioxidant and cytotoxicity activities. The formation of AgNPs was confirmed by UV-Vis spectroscopy at the wavelength of 420 nm (AgNP-L) and 430 nm (AgNP-S). The size were measured at 114 nm (AgNP-L) and 129 nm (AgNP-S) using a zetasizer. From TEM analysis, the size ranged from 10-300 nm and 10-180 nm for AgNP-L and AgNP-S with spherical shape. Zeta potentials recorded at -42.8 mV for AgNP-L and -43.9 mV for AgNP-S. XRD analysis matched the face-centered cubic (fcc) structure of silver. FTIR analysis revealed the presence of few functional groups of phenolic and flavonoid compounds which acts as the reducing agent in AgNPs synthesis. The AgNPs showed good inhibition against bacteria, but not fungus. Inhibition showed that highest against S. aureus with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition against E. coli with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in disc diffusion method. Same trend of result was reported for well diffusion method. In antioxidant assays, AgNP-L showed IC50 at 417.05 μg/mL and AgNP-S at 434.60 μg/mL (DPPH); 304.31 μg/mL (AgNP-L) and 326.83 μg/mL (AgNP-S) for ABTS, respectively.
format	Thesis
qualification_level	Master's degree
author	Che Mood, Che Nurul Azieyan
author_facet	Che Mood, Che Nurul Azieyan
author_sort	Che Mood, Che Nurul Azieyan
title	Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_short	Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_full	Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_fullStr	Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_full_unstemmed	Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_sort	silver nanoparticles of clinacanthus nutans aqueous extract: biosynthesis, phytochemical and selected biological activities
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2018
url	http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf
_version_	1747821849288376320

Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities

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