Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis
In the present study, with the main goal of mimicking the amidase activity of protease,artificial polymer catalyst and peptide catalyst were designed and synthesized followed by various evaluations to investigate the validity of the design to possess enzyme-like amidolytic catalysis. Poly(N-isopropy...
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2017
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my-usm-ep.479252020-11-23T02:00:44Z Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis 2017-11 Wong, Yoke Ming QH1 Natural history (General - Including nature conservation, geographical distribution) In the present study, with the main goal of mimicking the amidase activity of protease,artificial polymer catalyst and peptide catalyst were designed and synthesized followed by various evaluations to investigate the validity of the design to possess enzyme-like amidolytic catalysis. Poly(N-isopropylacrylamide) microgel(NMG) has been developed by adding various functional groups to control surface charges, hydrophobicity, pKaand protein adsorption capacitybut seldom for enzyme mimics. Here, NMGsanchored with three types of functional groups were developed and studied aspolymercatalyststo hydrolyze amide bonds under evaluated mild conditions. Various evaluationstrategies were testedfor efficient hydrolysis activity on a p-nitroaniline-based substrate by using a colorimetric assay. 2017-11 Thesis http://eprints.usm.my/47925/ http://eprints.usm.my/47925/1/DESIGN%20AND%20EVALUATION%20OF%20ARTIFICIAL.pdf%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Science) |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Wong, Yoke Ming Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| description |
In the present study, with the main goal of mimicking the amidase activity of protease,artificial polymer catalyst and peptide catalyst were designed and synthesized followed by various evaluations to investigate the validity of the design to possess enzyme-like amidolytic catalysis. Poly(N-isopropylacrylamide) microgel(NMG) has been developed by adding various functional groups to control surface charges, hydrophobicity, pKaand protein adsorption capacitybut seldom for enzyme mimics. Here, NMGsanchored with three types of functional groups were developed and studied aspolymercatalyststo hydrolyze amide bonds under evaluated mild conditions. Various evaluationstrategies were testedfor efficient hydrolysis activity on a p-nitroaniline-based substrate by using a colorimetric assay. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Wong, Yoke Ming |
| author_facet |
Wong, Yoke Ming |
| author_sort |
Wong, Yoke Ming |
| title |
Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| title_short |
Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| title_full |
Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| title_fullStr |
Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| title_full_unstemmed |
Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis |
| title_sort |
design and evaluation of artificial catalysts that possess enzyme-like amidolytic catalysis |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat (School of Biological Science) |
| publishDate |
2017 |
| url |
http://eprints.usm.my/47925/1/DESIGN%20AND%20EVALUATION%20OF%20ARTIFICIAL.pdf%20cut.pdf |
| _version_ |
1747821849773867008 |