Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana

Nowadays, CRISPR/Cas9 system has emerged as a powerful genome editing tool in various plant species, due to its simplicity and versatility. Typically, in plant system, Cas9 and sgNA are delivered into the plant cell in the form of DNA. However, the DNA delivery can be complicated with several prob...

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Main Author:	Ng, Kiaw Kiaw
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/47928/1/DEVELOPMENT%20OF%20A%20PEPTIDE-BASED.pdf%20cut.pdf
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id	my-usm-ep.47928
record_format	uketd_dc
spelling	my-usm-ep.479282020-11-23T02:27:05Z Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana 2017-12 Ng, Kiaw Kiaw QH1 Natural history (General - Including nature conservation, geographical distribution) Nowadays, CRISPR/Cas9 system has emerged as a powerful genome editing tool in various plant species, due to its simplicity and versatility. Typically, in plant system, Cas9 and sgNA are delivered into the plant cell in the form of DNA. However, the DNA delivery can be complicated with several problems, such as unexpected integration of exogenous DNA into plant genome, codon optimization and selection of promoter in respect to various plant species. To overcome these problems, a direct delivery system of Cas9:sgRNA complexes is needed for targeted genome editing in plants. Although there have been reports of the direct delivery Cas9:sgRNA complexes in protoplast and embryonic systems, there are no reports of the successful Cas9:sgRNA complexes delivery system in intact plant, owing to its hierarchical structures. Hence, the current study is aimed to develop a peptide-based delivery system for pre-assembled Cas9:sgRNA complexes into intact Arabidopsis thaliana for targeted genome editing. 2017-12 Thesis http://eprints.usm.my/47928/ http://eprints.usm.my/47928/1/DEVELOPMENT%20OF%20A%20PEPTIDE-BASED.pdf%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Science)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Ng, Kiaw Kiaw Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
description	Nowadays, CRISPR/Cas9 system has emerged as a powerful genome editing tool in various plant species, due to its simplicity and versatility. Typically, in plant system, Cas9 and sgNA are delivered into the plant cell in the form of DNA. However, the DNA delivery can be complicated with several problems, such as unexpected integration of exogenous DNA into plant genome, codon optimization and selection of promoter in respect to various plant species. To overcome these problems, a direct delivery system of Cas9:sgRNA complexes is needed for targeted genome editing in plants. Although there have been reports of the direct delivery Cas9:sgRNA complexes in protoplast and embryonic systems, there are no reports of the successful Cas9:sgRNA complexes delivery system in intact plant, owing to its hierarchical structures. Hence, the current study is aimed to develop a peptide-based delivery system for pre-assembled Cas9:sgRNA complexes into intact Arabidopsis thaliana for targeted genome editing.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ng, Kiaw Kiaw
author_facet	Ng, Kiaw Kiaw
author_sort	Ng, Kiaw Kiaw
title	Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
title_short	Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
title_full	Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
title_fullStr	Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
title_full_unstemmed	Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana
title_sort	development of a peptide-based system for delivery of cas9:sgrna complexes into arabidopsis thaliana
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Science)
publishDate	2017
url	http://eprints.usm.my/47928/1/DEVELOPMENT%20OF%20A%20PEPTIDE-BASED.pdf%20cut.pdf
_version_	1747821850511015936

Development Of A Peptide-Based System For Delivery Of Cas9:Sgrna Complexes Into Arabidopsis Thaliana

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