The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells

The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells

Cancer stem cells (CSCs) represent a small subpopulation within a tumour that possesses the stem-like properties but are also initiating resistance towards cytotoxic agent which contribute to cancer relapse. A natural compound such as curcumin that contains high polyphenol has been found to possess...

Full description

Saved in:
Bibliographic Details
Main Author: Satar, Nazilah Abdul
Format: Thesis
Language:English
Published: 2018
Subjects:
RZ Other systems of medicine
Online Access:http://eprints.usm.my/47955/1/NAZILAH%20BINTI%20ABDUL%20SATAR%20-%20THE%20CHEMO-SENSITISATION%20EFFECT%20OF.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.47955
record_format uketd_dc
spelling my-usm-ep.479552020-12-02T03:17:03Z The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells 2018-09 Satar, Nazilah Abdul RZ Other systems of medicine Cancer stem cells (CSCs) represent a small subpopulation within a tumour that possesses the stem-like properties but are also initiating resistance towards cytotoxic agent which contribute to cancer relapse. A natural compound such as curcumin that contains high polyphenol has been found to possess chemo-sensitivity effect with an ability to sensitise the CSCs to the cytotoxic agents such as cisplatin. Therefore, the present study was designed to investigate the efficiency of curcumin as a chemo-sensitiser in lung CSCs and to analyse its inhibitory effect on CSCs activity using A549 and H2170 cell lines that belong to non-small cell lung cancer (NSCLC). The NSCLC cell lines cultures were treated with various concentrations of cisplatin and curcumin to obtain the inhibitory concentration (IC50) using 3-(4, 5-dimethylthiazol-2-yl)-2H-tetrazolium, inner salt (MTS) assay. The lung CSCs with phenotype CD166+EpCAM+ was isolated using fluorescence-activated cell sorting (FACS). The efficiency of curcumin to sensitise lung CSCs was observed on apoptosis and stemness characteristics including migration ability, colonies and spheroid formation. The mRNA level was analysed for genes involved in apoptosis and stemness using quantitative real time-polymerase chain reaction (RT-qPCR). Liquid chromatography mass spectrometry (LCMS) was used to evaluate the effect of curcumin on CSC niche. The results discovered that exposure of NSCLC cell lines to curcumin (10 μM – 40 μM) reduced the percentage of cells viability to an average of ~51% and ~54% in both A549 and H2170. 2018-09 Thesis http://eprints.usm.my/47955/ http://eprints.usm.my/47955/1/NAZILAH%20BINTI%20ABDUL%20SATAR%20-%20THE%20CHEMO-SENSITISATION%20EFFECT%20OF.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RZ Other systems of medicine
spellingShingle RZ Other systems of medicine
Satar, Nazilah Abdul
The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
description Cancer stem cells (CSCs) represent a small subpopulation within a tumour that possesses the stem-like properties but are also initiating resistance towards cytotoxic agent which contribute to cancer relapse. A natural compound such as curcumin that contains high polyphenol has been found to possess chemo-sensitivity effect with an ability to sensitise the CSCs to the cytotoxic agents such as cisplatin. Therefore, the present study was designed to investigate the efficiency of curcumin as a chemo-sensitiser in lung CSCs and to analyse its inhibitory effect on CSCs activity using A549 and H2170 cell lines that belong to non-small cell lung cancer (NSCLC). The NSCLC cell lines cultures were treated with various concentrations of cisplatin and curcumin to obtain the inhibitory concentration (IC50) using 3-(4, 5-dimethylthiazol-2-yl)-2H-tetrazolium, inner salt (MTS) assay. The lung CSCs with phenotype CD166+EpCAM+ was isolated using fluorescence-activated cell sorting (FACS). The efficiency of curcumin to sensitise lung CSCs was observed on apoptosis and stemness characteristics including migration ability, colonies and spheroid formation. The mRNA level was analysed for genes involved in apoptosis and stemness using quantitative real time-polymerase chain reaction (RT-qPCR). Liquid chromatography mass spectrometry (LCMS) was used to evaluate the effect of curcumin on CSC niche. The results discovered that exposure of NSCLC cell lines to curcumin (10 μM – 40 μM) reduced the percentage of cells viability to an average of ~51% and ~54% in both A549 and H2170.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Satar, Nazilah Abdul
author_facet Satar, Nazilah Abdul
author_sort Satar, Nazilah Abdul
title The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
title_short The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
title_full The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
title_fullStr The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
title_full_unstemmed The Chemo-Sensitisation Effect Of Curcumin Against Low Dose Cisplatin On Non-Small Cell Lung Cancer (Nsclc) Stem Cells
title_sort chemo-sensitisation effect of curcumin against low dose cisplatin on non-small cell lung cancer (nsclc) stem cells
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2018
url http://eprints.usm.my/47955/1/NAZILAH%20BINTI%20ABDUL%20SATAR%20-%20THE%20CHEMO-SENSITISATION%20EFFECT%20OF.pdf
_version_ 1747821856364167168

Similar Items